Recruitment and establishment of the gut microbiome in arctic shorebirds
Recruitment and establishment of the gut microbiome in arctic shorebirds
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DOI：
10.1093/femsec/fix142
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发表时间：
2017-12-01
影响因子：
4.2
通讯作者：
Sandercock, Brett K.
中科院分区：
文献类型：
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作者：
Grond, Kirsten;Lanctot, Richard B.;Sandercock, Brett K.
肠道微生物区系在宿主健康中起着关键作用。哺乳动物在出生时获得肠道微生物区系，但鸟类肠道微生物招募的时间尚不清楚。我们评估了来自三种北极繁殖滨鸟的早熟雏鸟在孵化前或孵化后是否获得肠道微生物区系，然后记录了肠道微生物区系积累的速度和组成动态。与早先关于鸡孵化前微生物招募的报道相反，定量PCR和Illumina序列数据显示，滨鸟胚胎孵化前肠道中的微生物群可以忽略不计。对雏鸡粪便的分析表明，孵化后0-2天，肠道细菌丰度呈指数级增加，然后趋于稳定。肠道群落具有随机招募和向梭状芽胞杆菌和伽马变形杆菌为主的群落收敛的特点。我们的结论是，滨鸟雏鸟的肠道在孵化前可能没有微生物区系，但稳定的肠道微生物群早在3日龄就建立起来了，可能是环境接种造成的。
Gut microbiota play a key role in host health. Mammals acquire gut microbiota during birth, but timing of gut microbial recruitment in birds is unknown. We evaluated whether precocial chicks from three species of arctic-breeding shorebirds acquire gut microbiota before or after hatching, and then documented the rate and compositional dynamics of accumulation of gut microbiota. Contrary to earlier reports of microbial recruitment before hatching in chickens, quantitative PCR and Illumina sequence data indicated negligible microbiota in the guts of shorebird embryos before hatching. Analyses of chick feces indicated an exponential increase in bacterial abundance of guts 0-2 days post-hatch, followed by stabilization. Gut communities were characterized by stochastic recruitment and convergence towards a community dominated by Clostridia and Gammaproteobacteria. We conclude that guts of shorebird chicks are likely void of microbiota prior to hatch, but that stable gut microbiome establishes as early as 3 days of age, probably from environmental inocula.