Creatine supplementation to total parenteral nutrition improves creatine status and supports greater liver and kidney protein synthesis in neonatal piglets
Creatine supplementation to total parenteral nutrition improves creatine status and supports greater liver and kidney protein synthesis in neonatal piglets
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DOI：
10.1038/pr.2017.208
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发表时间：
2018-01-01
影响因子：
3.6
通讯作者：
Brunton, Janet A.
中科院分区：
文献类型：
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作者：
Dinesh, O. Chandani;Bertolo, Robert F.;Brunton, Janet A.
背景：肌酸不包含在商业儿科注射产品中；在肠外营养 (PN) 期间，必须通过精氨酸从头合成来满足全部肌酸需求。新生儿 PN 期间精氨酸状态不佳很常见，这可能会影响肌酸的积累。我们假设补充肌酸将改善 PN 喂养仔猪的肌酸状态并保留精氨酸。方法：对仔猪（3-5 日龄）进行 PN 喂养，添加或不添加肌酸 14 天。测量了肌酸代谢物的组织浓度和肌酸合成酶的活性，以及组织蛋白合成率和肝脏脂质参数。结果：提供肌酸降低了肾脏和胰腺的 L-精氨酸：甘氨酸脒基转移酶（AGAT，EC 号 2.1.4.1）活性和血浆胍基乙酸（GAA）浓度，表明肌酸从头合成的下调。肌酸可将血浆肌酸浓度提高至母猪饲喂参考水平，并提高大多数组织中的肌酸浓度，但不会提高大脑中的肌酸浓度。 PN 肌酸会导致肝脏和肾脏中的蛋白质合成增加，但不会导致胰腺、骨骼肌或肠道的蛋白质合成增加。补充肌酸还可以降低肝脏胆固醇浓度，但不会降低甘油三酯或总脂肪。结论：在 PN 中添加肌酸可以优化肌酸的积累，并减轻快速生长的新生儿肌酸合成的代谢负担。
BACKGROUND: Creatine is not included in commercial pediatric parenteral products; the entire creatine requirement must be met by de novo synthesis from arginine during parenteral nutrition (PN). Poor arginine status is common during PN in neonates, which may compromise creatine accretion. We hypothesized that creatine supplementation will improve creatine status and spare arginine in PN-fed piglets.METHODS: Piglets (3-5-day (d) old) were provided PN with or without creatine for 14 d. Tissue concentrations of creatine metabolites and activities of creatine-synthesizing enzymes, as well as tissue protein synthesis rates and liver lipid parameters, were measured.RESULTS: Creatine provision lowered kidney and pancreas L-arginine: glycine amidinotransferase (AGAT, EC number 2.1.4.1) activities and plasma guanidinoacetic acid (GAA) concentration, suggesting the downregulation of de novo creatine synthesis. Creatine increased plasma creatine concentrations to sow-fed reference levels and increased the creatine concentrations in most tissues, but not in the brain. PN creatine resulted in greater protein synthesis in the liver and the kidney, but not in the pancreas, skeletal muscle, or gut. Creatine supplementation also reduced liver cholesterol concentrations, but not triglyceride or total fat.CONCLUSION: The addition of creatine to PN may optimize the accretion of creatine and reduce the metabolic burden of creatine synthesis in rapidly growing neonates.