Aluminum oxide nanoparticles mediated toxicity, loss of appendages in progeny of Drosophila melanogaster on chronic exposure
Aluminum oxide nanoparticles mediated toxicity, loss of appendages in progeny of Drosophila melanogaster on chronic exposure
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DOI：
10.1080/17435390.2019.1602680
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发表时间：
2019-04-20
期刊：
影响因子：
5
通讯作者：
Kohli, Ekta
中科院分区：
文献类型：
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作者：
Anand, Avnika Singh;Gahlot, Urmila;Kohli, Ekta
氧化铝（Al2O3）纳米颗粒（NPs）具有广泛的应用，其导致有意和无意的暴露于人类，使得理解纳米生物相互作用变得重要。在这项研究中，我们做了尝试，以评估铝纳米粒子慢性暴露对果蝇的毒性效应。苍蝇暴露于浓度为0.1和1mM的Al2O3纳米颗粒通过摄入在其整个生命周期和后代苍蝇进行筛选的行为和表型异常。通过幼虫爬行、苍蝇攀爬和两次味觉测试记录苍蝇的行为异常。长期暴露于Al2O3纳米颗粒导致果蝇的附肢丧失，导致五条腿果蝇、四条腿果蝇和无笼头果蝇。暴露于Al2O3纳米颗粒导致苍蝇肾衰竭，如腹部肿胀所观察到的。我们的观察清楚地表明，这些纳米颗粒可能会导致有害的健康疾病，这些疾病与人类出生畸形和肾衰竭有关。通过蛋白质组学分析研究了细胞水平的损伤。307独特的蛋白质表达暴露于氧化铝纳米颗粒和51蛋白质差异表达。差异表达蛋白的富集分析表明，横纹肌细胞分化，消化道形态发生，光转导，染色质组织和DNA双链体解旋的调节显着改变。
Aluminum oxide (Al2O3) nanoparticles (NPs) have a wide number of applications which cause intentional and unintentional exposure to humans, making it important to understand the nano-bio interaction. In this study, we made an attempt to evaluate the toxic effects of Al2O3 NPs chronic exposure on Drosophila melanogaster. Flies were exposed to Al2O3 NPs at concentration 0.1 and 1mM via ingestion throughout their lifespan and progeny flies were screened for behavioral and phenotypic abnormalities. Behavioral abnormalities in flies were recorded through larval crawling, climbing in flies and two taste testing. Chronic exposure of Al2O3 NPs resulted in the loss of appendages in flies resulting in five legs flies, four legs flies and absence of haltere. Exposure to Al2O3 NPs caused renal failure in flies as observed by swollen abdomen. Our observations clearly showed that these NPs could cause detrimental health ailments which relate to human birth deformities and kidney failure. Damage at the cellular level was studied through proteomic profiling. Three hundred and seven unique proteins were expressed on exposure to Al2O3 NPs and 51 proteins were differentially expressed. Enrichment analysis of differentially expressed proteins showed significant alteration in striated muscle cell differentiation, digestive tract morphogenesis, phototransduction, regulation of chromatin organization and DNA duplex unwinding.