Colloidal gold-labeled insulin complex. Characterization and binding to adipocytes.
Colloidal gold-labeled insulin complex. Characterization and binding to adipocytes.
复制标题
胶体金标记的胰岛素复合物。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
E. Pfeiffer
中科院分区：
文献类型：
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作者：
U. Moll;C. Thun;E. Pfeiffer
利用生物活性胰岛素金配合物作为超微结构标记物，研究大鼠离体脂肪细胞中胰岛素的结合位点、摄取和内化。报道了单分散颗粒的制备条件，该颗粒直径约16 nm，装载约100个胰岛素分子。该综合体至少在六周内是稳定的。单个颗粒或小团簇散布在细胞膜上。未结合受体的分布似乎独立于脂肪细胞中预先存在的表面连接囊泡的广泛系统。颗粒的摄取主要通过无包被的胞浆内陷发生；包覆网格蛋白的凹坑在这个过程中似乎并不重要。溶酶体样结构含有10-15个颗粒的聚集体。这些数据表明，胰岛素金配合物是胰岛素结合位点特异性标记的有用标记物。
Biologically active insulin gold complex was used as an ultrastructural marker to study insulin binding sites, uptake, and internalization in isolated rat adipocytes. The preparation conditions for monodispersed particles, ca. 16 nm in diameter and loaded with approximately 100 insulin molecules, are reported. The complex is stable for at least six weeks. Single particles or small clusters were scattered across the cell membrane. The distribution of unbound receptors seemed to be independent of the extensive system of pre-existing surface connected vesicles in adipocytes. The uptake of particles took place predominantly via non-coated pinocytotic invaginations; clathrin-coated pits did not seem to be important for this process. Lysosome-like structures contained aggregates of 10-15 particles. These data suggest that insulin gold complex is a useful marker for the specific labeling of insulin binding sites.