Translation of merD in Tn21.
Translation of merD in Tn21.
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Tn21 中 merD 的翻译。
DOI:
10.1128/jb.171.4.2222-2225.1989
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发表时间:
1989
影响因子:
3.2
通讯作者:
Summers,AO
中科院分区:
文献类型:
--
作者:
Lee,IW;Gambill,BD;Summers,AO
All four sequenced examples of the mercury resistance (mer) operon of gram-negative bacteria have a promoter-distal reading frame, merD, whose removal has little effect on the resistance phenotype and whose translation has not previously been observed. Using merD-lacZ protein fusions, we show that merD is translated. However, Hg(II)-induced merD expression, as measured by beta-galactosidase activity and immunoblotting, is 10- to 15-fold lower than that of fusions to the gene immediately preceding it, merA.