Interleukin-17 gene expression in patients with rheumatoid arthritis
Interleukin-17 gene expression in patients with rheumatoid arthritis
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DOI：
10.1007/s10165-007-0015-y
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发表时间：
2008-02-01
影响因子：
2.2
通讯作者：
Sumida, Takayuki
中科院分区：
文献类型：
--
作者：
Kohno, Mika;Tsutsumi, Akito;Sumida, Takayuki
白介素17是一种促炎细胞因子。最近的动物研究表明，IL-17在关节炎的发生和发展中起着重要作用。然而，IL-17是否在人类类风湿性关节炎(RA)中起着重要作用仍不清楚。在这里，我们研究了IL-17在类风湿关节炎患者中的作用。从RA(n=11)和骨关节炎(OA，n=10)患者的膝关节滑膜组织和RA(n=52)和健康人(n=34)的PBMC中提取c DNA。采用实时定量聚合酶链式反应检测IL-17基因表达水平，并与不同临床参数进行比较。类风湿关节炎滑膜组织中IL-17基因表达与骨性关节炎相似。RA患者PBMC中IL-17基因表达水平显著高于对照组。抗肿瘤坏死因子-α阻滞剂(英夫利昔单抗或依那西普)治疗两周后的反应(DAS的变化)与IL-17基因表达水平的变化无关。治疗应答者在基线(治疗前)的IL-17/肿瘤坏死因子-α基因表达比率趋于较低。IL-17基因在PBMC中的表达可能与RA的炎症过程有关。IL-17/TNF-α表达比值是反映抗-TNF-α治疗反应的一个有潜力的指标。
Interleukin-17 is a proinflammatory cytokine. Recent animal studies have shown that IL-17 plays a role in the initiation and progression of arthritis. However, whether IL-17 has a prominent role in human rheumatoid arthritis (RA) or not remains unclear. Here we investigated the role of IL-17 in patients with RA. cDNA was prepared from knee joint synovial tissues of RA (n = 11) and osteoarthritic (OA, n = 10) patients and PBMC of RA (n = 52) and healthy subjects (n = 34). IL-17 gene expression level was measured by real-time PCR, and was compared with various clinical parameters. IL-17 gene expression in synovial tissues of RA was similar to that in OA. IL-17 gene expression level in PBMC of RA patients was significantly higher than in the control. The response (changes in DAS) to two-week treatment with anti-TNF-alpha blockers (infliximab or etanercept) did not correlate with changes in IL-17 gene expression levels. The IL-17/TNF-alpha gene expression ratio at baseline (before treatment) tended to be lower in responders to the treatment. Expression of IL-17 gene in PBMC may be associated with the inflammatory process of RA. IL-17/TNF-alpha expression ratio is a potentially suitable marker of response to anti-TNF-alpha therapy.