CHO cell repair of single-strand and double-strand DNA breaks induced by gamma- and alpha-radiations.
CHO cell repair of single-strand and double-strand DNA breaks induced by gamma- and alpha-radiations.
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CHO 细胞修复由 γ 和 α 辐射引起的单链和双链 DNA 断裂。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
Cooper Wg
中科院分区：
文献类型：
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作者：
A. Cole;F. Shonka;P. Corry;Cooper Wg
使用中性和碱性蔗糖梯度沉降分析来测量暴露于 γ 或 α 辐射的中国仓鼠卵巢 (CHO) 细胞 DNA 中的双链和单链断裂。照射后，将细胞孵育15-180分钟以测试细胞重新连接DNA断裂的能力。对于由低于 20 krad 的 γ 或 α 剂量诱导的单链断裂和由低于 60 krad 的 γ 剂量诱导的双链断裂，观察到基本上完全的重新连接。对于低于 40 krad 的 α 剂量诱导的双链断裂，观察到大约 80% 的重新连接。在较高剂量下，修复系统似乎饱和，基本上没有额外的断裂被重新连接。
Neutral and alkaline sucrose gradient sedimentation analysis was used to measure double- and single-strand breaks in the DNA of Chinese hamster ovary (CHO) cells exposed to either gamma- or alpha-radiation. After irradiation, cells were incubated for 15-180 min to test the ability of the cell to rejoin the DNA breaks. Essentially complete rejoining was observed for single-strand breaks induced by gamma- or alpha-doses below 20 krad and for double-strand breaks induced by gamma doses below 60 krad. Approximately 80% rejoining was observed for double-strand breaks induced by alpha doses below 40 krad. At higher doses, the repair system appeared to saturate in such a way that essentially no additional breaks were rejoined.