The relative abundance of wheat Rubisco activase isoforms is post-transcriptionally regulated.
The relative abundance of wheat Rubisco activase isoforms is post-transcriptionally regulated.
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DOI：
10.1007/s11120-021-00830-6
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发表时间：
2021-05
影响因子：
3.7
通讯作者：
Carmo-Silva E
中科院分区：
文献类型：
--
作者：
Perdomo JA;Buchner P;Carmo-Silva E
昼夜节律和光的可用性影响转录-翻译反馈回路，调节光合蛋白的合成。CO2固定酶Rubisco是主要作物物种叶片中最丰富的蛋白质，其活性取决于与分子伴侣Rubisco活化酶（Rca）的相互作用。小麦（Triticum aestivum L.）（小麦）中，存在三种Rca同种型，其调节特性不同。在这里，我们测试的假设，氧化还原敏感和氧化还原不敏感的Rca亚型的相对丰度可以在整个光暗昼夜周期在小麦差异调节。虽然TaRca 1-β表达在一天中始终可以忽略不计，但TaRca 2-β和TaRca 2-α的转录水平较高，并在一天开始时增加，峰值水平出现在光周期的中间。TaRca-β蛋白在TaRca-2-β表达高峰后1.5 h出现最大量的下降，而TaRca-α的丰度在整个光周期内保持恒定。氧化还原敏感性TaRca-α亚型的丰度较低，在转录水平上占氧化还原不敏感性TaRca-β的85%，在蛋白水平上占12.5%。Rubisco的大小亚基基因的表达在整个昼夜周期中没有表现出一致的模式，但在完全展开的小麦叶片中，Rubisco的丰度在黑暗期间下降了20%。这些结果，结合缺乏Rca亚型和Rubisco在整个昼夜循环的转录和蛋白质丰度之间的相关性，表明这些光合酶的丰度是转录后调节。在线版本包含补充材料，可通过10.1007/s11120-021-00830-6获得。
Diurnal rhythms and light availability affect transcription–translation feedback loops that regulate the synthesis of photosynthetic proteins. The CO2-fixing enzyme Rubisco is the most abundant protein in the leaves of major crop species and its activity depends on interaction with the molecular chaperone Rubisco activase (Rca). In Triticum aestivum L. (wheat), three Rca isoforms are present that differ in their regulatory properties. Here, we tested the hypothesis that the relative abundance of the redox-sensitive and redox-insensitive Rca isoforms could be differentially regulated throughout light–dark diel cycle in wheat. While TaRca1-β expression was consistently negligible throughout the day, transcript levels of both TaRca2-β and TaRca2-α were higher and increased at the start of the day, with peak levels occurring at the middle of the photoperiod. Abundance of TaRca-β protein was maximal 1.5 h after the peak in TaRca2-β expression, but the abundance of TaRca-α remained constant during the entire photoperiod. The redox-sensitive TaRca-α isoform was less abundant, representing 85% of the redox-insensitive TaRca-β at the transcript level and 12.5% at the protein level. Expression of Rubisco large and small subunit genes did not show a consistent pattern throughout the diel cycle, but the abundance of Rubisco decreased by up to 20% during the dark period in fully expanded wheat leaves. These results, combined with a lack of correlation between transcript and protein abundance for both Rca isoforms and Rubisco throughout the entire diel cycle, suggest that the abundance of these photosynthetic enzymes is post-transcriptionally regulated. The online version contains supplementary material available at 10.1007/s11120-021-00830-6.
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影响因子：
7.3
作者：
Davies, B. N.;Griffiths, H.
通讯作者：
Griffiths, H.
影响因子：
6.9
作者：
Esquivel, MG;Ferreira, RB;Teixeira, AR
通讯作者：
Teixeira, AR
影响因子：
7.2
作者：
Degen, Gustaf E.;Worrall, Dawn;Carmo-Silva, Elizabete
通讯作者：
Carmo-Silva, Elizabete
影响因子：
6.8
作者：
AKAIKE, H
通讯作者：
AKAIKE, H
影响因子：
4.3
作者：
DeRidder, Benjamin P.;Shybut, Mikel E.;Shapiro, Mariya B.
通讯作者：
Shapiro, Mariya B.