IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES .2. INFLUENCE OF SERUM ON GRANULE FORMATION HYDROLASE PRODUCTION AND PINOCYTOSIS
IN VITRO DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES .2. INFLUENCE OF SERUM ON GRANULE FORMATION HYDROLASE PRODUCTION AND PINOCYTOSIS
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DOI：
10.1084/jem.121.5.835
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发表时间：
1965-01-01
影响因子：
15.3
通讯作者：
BENSON, B
中科院分区：
文献类型：
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作者：
COHN, ZA;BENSON, B
培养基中新生牛犊血清浓度对小鼠单核吞噬细胞形态和生化特性有显著影响。在低血清浓度下，细胞形成少量微小的细胞质颗粒，酸性磷酸酶、组织蛋白酶和[β]-葡萄糖醛酸苷酶很少或没有增加。随着血清浓度的升高，颗粒形成速度加快，颗粒大小增大。血清水平越高，三种水解酶的生成速率和总量越高。对活细胞的观察表明，积聚在核周区的相密颗粒来源于胞泡。这些透明的囊泡融合并迁移到中心球，在那里它们经历了相密度的逐渐增加，并与酸性磷酸酶积极反应。描述了一种用于评价胞饮过程的显微技术[长虚线]方法。当采用这种方法时，随着培养基中犊牛血清浓度的升高，胞饮率呈曲线上升。比较了牛、马和兔血清对小鼠细胞的影响。提示胞饮作用是小鼠巨噬细胞颗粒形成和水解酶产生的主要调节因子。
The concentration of newborn calf serum in the medium has marked effects on the morphological and biochemical properties of mouse mononuclear phagocytes. At a low serum concentration, the cells developed small numbers of tiny cytoplasmic granules and little or no increase in acid phosphatase, cathepsin, and [beta]-glucuronidase. As the serum concentration was raised, granules were formed at a more rapid rate and were larger in size. The rate of production and total amount of three hydrolytic enzymes was increased at higher levels of serum. Observations on living cells indicated that the phase-dense granules which accumulated in the perinuclear region were derived from pinocytic vesicles. These clear vesicles fused and migrated to the centrosphere where they underwent a gradual increase in phase density and reacted positively for acid phosphatase. A microscopic tech [long dash] nique was described for the evaluation of the pinocytic process. When this method was employed, the rate of pinocytosis increased curvilinear-ly with elevations in the calf serum concentration of the medium. The comparative influence of bovine, horse, and rabbit serum on mouse cells was evaluated. It is suggested that pinocytosis is a major regulator of granule formation and hydrolytic enzyme production by the mouse macrophage.