Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine
Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine
复制标题
Oct4和Sox2过表达可促进小眉山猪骨髓间充质干细胞的增殖和分化。
DOI：
10.4238/2013.december.2.5
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发表时间：
2013-01-01
影响因子：
0.4
通讯作者：
Wang, F.
中科院分区：
文献类型：
--
作者：
Fan, Y. X.;Gu, C. H.;Wang, F.
骨髓间充质干细胞（BMSCs）是一种自我更新的多能细胞，能够分化成各种细胞系，广泛应用于组织工程和细胞治疗。近年来，涉及骨髓间充质干细胞的临床研究越来越受欢迎。为了进行适当的研究，首先需要在体外扩增大量功能性骨髓间充质干细胞。然而，经过几代体外扩增后，骨髓间充质干细胞的增殖和分化潜能逐渐下降。为了确定Oct4或Sox2的过表达是否可能阻止这种下降，我们用慢病毒将胚胎干细胞多能性和自我更新所必需的Oct4或Sox2转染到小梅山猪的骨髓间充质干细胞中。结果表明，在含有碱性成纤维细胞生长因子的培养基中，过表达Sox2或Oct4骨髓间充质干细胞比对照细胞具有更高的增殖和分化能力，提示对干细胞相关基因进行遗传修饰是维持骨髓间充质干细胞增殖和分化潜能的有效途径。
Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to conduct appropriate research, it is first necessary to amplify large amounts of functional BMSCs in vitro. However, after several passages of expanding in vitro, the proliferation and differentiation potential of BMSCs gradually decline. To determine whether overexpression of Oct4 or Sox2 might prevent this decline, we transfected Oct4 or Sox2, which are essential for the pluripotency and self-renewal of embryonic stem cells, into BMSCs of Xiaomeishan porcine by a lentivirus. The results showed that overexpression of Sox2 or Oct4 BMSCs in culture media containing a basic fibroblast growth factor resulted in higher proliferation and differentiation compared to controls, suggesting that genetic modification of stemness-related genes is an efficient way to maintain the proliferation and differentiation potential of BMSCs.