In vitro studies of baicalin alone or in combination with Salvia miltiorrhiza extract as a potential anti-cancer agent.
In vitro studies of baicalin alone or in combination with Salvia miltiorrhiza extract as a potential anti-cancer agent.
复制标题
DOI：
10.3892/ijo.26.1.217
复制
发表时间：
2005
影响因子：
5.2
通讯作者：
K. Franek;Zengtong Zhou;Wei-Dong Zhang;Wen Chen
中科院分区：
文献类型：
--
作者：
K. Franek;Zengtong Zhou;Wei-Dong Zhang;Wen Chen
几个世纪以来一直用于疾病预防和治疗的传统中国草药（TCM）正在被用作西方癌症疗法的替代品。从一组中药中，我们选择了代表两类功能植物的四种化合物，纯化的植物黄素黄芩素（循环刺激剂）和黄芩苷（解热剂），以及纯化的丹参提取物（SM-470，循环刺激剂）和山茶提取物（Cam-300，解热剂），并研究了它们对人乳腺癌细胞系MCF-7和T-47D的抗增殖作用。4种化合物均能抑制MCF-7和T-47D细胞的增殖，其中黄芩苷的抑制作用最强。此外，来自不同类别的化合物的组合提供了增强的潜在治疗益处；SM-470与黄芩苷、Cam-300或黄芩苷联用可增强对细胞增殖的抑制作用。SM-470与黄芩苷共同作用对MCF-7细胞增殖有协同抑制作用。相比之下，同一组合对T-47D细胞增殖的抑制仅依赖于黄芩苷。这些化合物的抗增殖作用可以扩展到其他类型的癌症；人头颈癌上皮细胞系CAL-27和FaDu对4种药物均有敏感反应。SM-470、Cam-300、黄芩苷和黄芩苷对人乳腺癌细胞和CAL-27、FaDu细胞的增殖均有不同程度的抑制作用。黄芩苷与SM-470联用可产生加性效应，提示其作用机制可能不同。T-47D、MCF-7和FaDu细胞可能有助于探索黄芩苷和SM-470作用的细胞和分子机制。
Traditional Chinese herbal medicines (TCM) that for centuries have been used in disease prevention and treatment are finding use as alternatives to Western cancer therapies. From a panel of TCM, we chose four compounds representing two functional classes of botanicals, the purified plant flavins scutellarin (a circulatory stimulant) and baicalin (antipyretic), and two extracts purified from Salvia miltiorrhiza (SM-470, circulatory stimulant) and Camellia sinensis (Cam-300, antipyretic), and examined their anti-proliferation effects on the human breast cancer cell lines MCF-7 and T-47D. All four compounds inhibited MCF-7 and T-47D cell proliferation, baicalin being the most potent inhibitor. Moreover, the combination of compounds from different classes offers enhanced potential therapeutic benefits; the combination of SM-470 with scutellarin, Cam-300 or baicalin, augmented the inhibition of cell proliferation. A synergistic inhibitory effect on MCF-7 cell proliferation was also observed when SM-470 and baicalin were applied together. In contrast, inhibition of T-47D cell proliferation using the same combination was dependent on baicalin only. The anti-proliferative effects of these compounds can be extended to other cancer types; the human head and neck cancer epithelial cell lines CAL-27 and FaDu were also sensitive to the four drugs. Overall, SM-470, Cam-300, scutellarin and baicalin inhibited the proliferation of human breast cancer cells and CAL-27 and FaDu cells with different potency. Baicalin and SM-470 in combination produced additive effects, suggesting these compounds may function by different mechanisms. T-47D, MCF-7, and FaDu cells may be useful in exploring the cellular and molecular mechanisms of action of baicalin and SM-470.