Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells
Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells
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DOI：
10.1016/j.aca.2022.339926
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发表时间：
2022-05-20
影响因子：
6.2
通讯作者：
Fujimaki, Makoto
中科院分区：
文献类型：
--
作者：
Ashiba, Hiroki;Yasuura, Masato;Fujimaki, Makoto
利用亚皮升微孔开发了快速和灵敏的病毒检测方法。一种方法使用聚集诱导发射(AIE)试剂，另一种方法使用高浓度为10(8)粒子/毫升的免疫磁珠支持的酶反应。使用AIE试剂检测甲型流感病毒的检测限值为3×10(5)拷贝/毫升，在总测量时间的1分钟内即可达到。高浓度免疫磁珠法的检测下限低至1×10(2)拷贝/毫升。所开发的方法是超高速、超灵敏病毒检测的有效和实用工具。
Quick and sensitive virus detection methods have been developed using sub-picoliter microwells. One method uses an aggregation-induced emission (AIE) reagent, and the other uses an enzymatic reaction supported with immunomagnetic beads at a high concentration of 10(8) particles/mL. Examination of influenza A virus detection using the AIE reagent exhibited a detection limit of 3 x 10(5) copies/mL, which was achievable within 1 min of the total measurement time. The high-concentration immunomagnetic beads method exhibited a detection limit as low as 1 x 10(2) copies/mL. The developed methods are effective and practical tools for ultrafast and ultrasensitive virus detection.