ZINC AND ZINC LIGANDS IN HUMAN SEMINAL PLASMA .3. THE PRINCIPAL LOW-MOLECULAR WEIGHT ZINC LIGAND IN PROSTATIC SECRETION AND SEMINAL PLASMA
ZINC AND ZINC LIGANDS IN HUMAN SEMINAL PLASMA .3. THE PRINCIPAL LOW-MOLECULAR WEIGHT ZINC LIGAND IN PROSTATIC SECRETION AND SEMINAL PLASMA
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DOI：
10.1111/j.1748-1716.1982.tb10600.x
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
ARVER, S
中科院分区：
文献类型：
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作者：
ARVER, S
人前列腺液和精浆中锌的主要结合成分是可逆结合锌，并具有低分子量。用胰蛋白酶和蛋白酶或酸水解处理这种含锌复合物（LMW-Zn）不能改变其锌结合特性或凝胶色谱特征。柠檬酸裂解酶、苹果酸脱氢酶和乳酸脱氢酶联合去除柠檬酸时，柠檬酸与锌的结合能力完全丧失。在Sephadex G-25色谱柱上对前列腺液或精浆凝胶层析获得的低分子量锌制剂进行光谱扫描，其吸收曲线与扫描柠檬酸锌溶液获得的吸收曲线相同。将同样的分析应用于人类母乳中的低分子锌也得到了类似的结果。柠檬酸盐是前列腺液、精浆和母乳中主要的低毫瓦锌配体。
The principal Zn binding component in human prostatic fluid and seminal plasma was to bind Zn reversible and to be of low MW. Treatment of this Zn-containing complex (LMW-Zn) with trypsin and pronase or acid hydrolysis failed to alter its Zn binding properties or gel chromatographic characteristics. Removal of citrate with a combination of citrate lyase, malate dehydrogenase and lactate dehydrogenase did, however, completely abolish its ability to bind Zn. Spectrographical scanning of LMW-Zn preparations obtained by gel chromatography of prostatic fluid or seminal plasma on a Sephadex G-25 column gave identical absorption curves with those obtained by scanning of a Zn-citrate solution. Application of the same analysis to LMW-Zn of human breast milk yielded similar results. Citrate is the major low MW Zn ligand in prostatic fluid, seminal plasma and breast milk.