Impact of glycerol-3-phosphate dehydrogenase on virulence factor production by Pseudomonas aeruginosa
Impact of glycerol-3-phosphate dehydrogenase on virulence factor production by Pseudomonas aeruginosa
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DOI：
10.1139/cjm-2014-0485
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发表时间：
2014-12-01
影响因子：
2.8
通讯作者：
Silo-Suh, Laura
中科院分区：
文献类型：
--
作者：
Daniels, Jonathan B.;Scoffield, Jessica;Silo-Suh, Laura
铜绿假单胞菌通过利用各种适应策略在囊性纤维化(CF)肺中建立终生慢性感染。其中一些策略包括改变代谢途径，以利用宿主环境中随时可用的营养物质。呼吸道痰中含有铜绿假单胞菌可利用的各种宿主来源的营养物质，包括肺表面活性物质的主要成分磷脂酰胆碱。铜绿假单胞菌可以将磷脂酰胆碱降解为甘油和脂肪酸，以增加CF肺中可用碳源的利用率。在这项研究中，我们表明，一些对CF适应的铜绿假单胞菌比非适应分离株更有效地利用甘油作为碳源。此外，甘油利用所需基因的突变会影响铜绿假单胞菌急性和慢性分离株中几种毒力因子的产生。综上所述，这些结果表明，干扰这一代谢途径可能具有潜在的治疗益处。
Pseudomonas aeruginosa establishes life-long chronic infections in the cystic fibrosis (CF) lung by utilizing various adaptation strategies. Some of these strategies include altering metabolic pathways to utilize readily available nutrients present in the host environment. The airway sputum contains various host-derived nutrients that can be utilized by P. aeruginosa, including phosphatidylcholine, a major component of lung surfactant. Pseudomonas aeruginosa can degrade phosphatidylcholine to glycerol and fatty acids to increase the availability of usable carbon sources in the CF lung. In this study, we show that some CF-adapted P. aeruginosa isolates utilize glycerol more efficiently as a carbon source than nonadapted isolates. Furthermore, a mutation in a gene required for glycerol utilization impacts the production of several virulence factors in both acute and chronic isolates of P. aeruginosa. Taken together, the results suggest that interference with this metabolic pathway may have potential therapeutic benefits.