Diallyl trisulfide induces apoptosis in Jurkat cells by the modification of cysteine residues in thioredoxin.
Diallyl trisulfide induces apoptosis in Jurkat cells by the modification of cysteine residues in thioredoxin.
复制标题
二烯丙基三硫化物通过修饰硫氧还蛋白中的半胱氨酸残基诱导 Jurkat 细胞凋亡。
DOI：
10.1080/09168451.2014.921564
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Seki T.
中科院分区：
文献类型：
--
作者：
Watanabe K;Hosono T;Watanabe K;Hosono-Fukao T;Ariga T;Seki T.
本文报道了二烯丙基三硫化物(DATS)氧化修饰特定半胱氨酸残基(S)对蛋白质功能的调节。在本研究中，我们通过尿素-聚丙烯酰胺凝胶电泳法检测了DATS是否修饰了硫氧还蛋白的半胱氨酸残基(TRX)。DATS修饰了TRX中两个特定的半胱氨酸残基，这种半胱氨酸残基的氧化修饰可能是DATS诱导白血病细胞凋亡的唯一原因。
We reported the regulation of protein function by oxidative modification of the specific cysteine residue(s) by diallyl trisulfide (DATS). In this study, we examined if DATS modifies the cysteine residue of thioredoxin (Trx) by urea-polyacryl amide gel electrophoresis. DATS modified two specific cysteine residues in Trx and this oxidative modification of cysteine residues would be sole causative of the apoptosis induced by DATS in leukemic cells.