Possible association of oestrogen and Cryba4 with masticatory muscle tendon-aponeurosis hyperplasia
Possible association of oestrogen and Cryba4 with masticatory muscle tendon-aponeurosis hyperplasia
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DOI：
10.1111/odi.12876
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发表时间：
2019-01-01
期刊：
影响因子：
3.8
通讯作者：
Yoda, T.
中科院分区：
文献类型：
--
作者：
Hayashi, N.;Sato, T.;Yoda, T.
目的咀嚼肌肌腱-腱膜增生，伴张口受限，自青春期起发展缓慢。这种疾病在女性中的患病率高于男性，这表明雌激素的作用。由于经常观察到的功能习惯，机械应力可能参与了该疾病的发病和进展。为了阐明病理情况，我们以小鼠TT-D6细胞为实验对象，研究了雌激素对细胞功能的影响以及机械应力与晶体蛋白A4 （Cryba4）的关系。材料与方法进行细胞增殖实验、RT-PCR、实时RT-PCR、Western blot分析和机械加载实验。结果生理剂量的雌激素使TT-D6细胞的硬化蛋白和腱调节蛋白水平升高。相反，Cryba4的强制表达抑制了这些细胞中硬化轴的表达。令人惊讶的是，雌激素显著促进cryba4过表达TT-D6的细胞分化。此外，拉伸力诱导这些肌腱细胞中Cryba4的表达。结论雌激素和Cryba4可能与咀嚼肌肌腱-腱膜增生有关。
Objective Masticatory muscle tendon-aponeurosis hyperplasia, which is associated with limited mouth opening, progresses very slowly from adolescence. The prevalence rates of this disease are higher among women than among men, suggesting oestrogen involvement. As parafunctional habits are frequently observed, mechanical stress is likely involved in the pathogenesis and advancement of this disease. To elucidate the pathological condition, we examined the effect of oestrogen on tenocyte function and the relationship between mechanical stress and crystallin beta A4 (Cryba4), using murine TT-D6 tenocytes. Materials and Methods Cell proliferation assays, RT-PCR, real-time RT-PCR, Western blot analysis and mechanical loading experiments were performed. Results The physiological dose of oestrogen increased the levels of scleraxis and tenomodulin in TT-D6 tenocytes. In contrast, forced expression of Cryba4 inhibited scleraxis expression in these cells. Surprisingly, oestrogen significantly promoted cell differentiation in the Cryba4-overexpressing TT-D6 tenocytes. Moreover, tensile force induced Cryba4 expression in these tendon cells. Conclusion Oestrogen and Cryba4 may be associated with the progression of masticatory muscle tendon-aponeurosis hyperplasia.