A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse.
A novel and efficient model of coronary artery ligation and myocardial infarction in the mouse.
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DOI：
10.1161/circresaha.110.223925
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发表时间：
2010-12-10
影响因子：
20.1
通讯作者：
Koch WJ
中科院分区：
文献类型：
--
作者：
Gao E;Lei YH;Shang X;Huang ZM;Zuo L;Boucher M;Fan Q;Chuprun JK;Ma XL;Koch WJ
在小鼠中诱导心肌梗死（MI）的冠状动脉结扎通常通过需要通气和开胸（经典方法）的侵入性且耗时的方法进行，通常导致广泛的组织损伤和高死亡率。我们开发了一种新的和快速的手术方法来诱导心肌梗死，不需要通气。本研究的目的是发展和全面描述这种方法，并直接将其与经典方法进行比较。将雄性C57/B6小鼠分为四组：新方法MI（MI-N）或假手术（S-N）、经典方法MI（MI-C）或假手术（S-C）。在新方法中，通过小切口手动暴露心脏而无需插管并诱导MI。在经典的方法中，MI是通过开胸通气诱导的。在缺血/再灌注损伤模型中使用类似的组。这种新型MI手术快速，平均手术时间为1.22±0.05 min，而经典方法每次手术需要23.2±0.6 min。MI-N组的手术死亡率为3%，MI-C组为15.9%。MI-N组心律失常发生率显著降低。术后TNFα和髓过氧化物酶（MPO）水平较低，提示炎症反应较轻。MI后总体28天生存率为68%（MI-N）和48%（MI-C）。重要的是，两种方法之间的梗死面积或MI后心功能没有差异。与经典方法相比，这种新的小鼠MI快速方法代表了更有效和损伤更小的心肌缺血损伤模型。
Coronary artery ligation to induce myocardial infarction (MI) in mice is typically performed by an invasive and time consuming approach that requires ventilation and chest opening (classical method), often resulting in extensive tissue damage and high mortality. We developed a novel and rapid surgical method to induce MI that does not require ventilation. The purpose of this study was to develop and comprehensively describe this method and directly compare it to the classical method. Male C57/B6 mice were grouped into four groups: new method MI (MI-N) or sham (S-N), classical method MI (MI-C) or sham (S-C). In new method, heart was manually exposed without intubation through a small incision and MI was induced. In classical method, MI was induced through a ventilated thoracotomy. Similar groups were used in an ischemia/reperfusion injury model. This novel MI procedure is rapid with an average procedure time of 1.22±0.05 min while the classical method requires 23.2±0.6 min per procedure. Surgical mortality was 3% in MI-N and 15.9% in MI-C. The rate of arrhythmia was significantly lower in MI-N. The post-surgical levels of TNFα and myeloperoxidase (MPO) were lower in new method indicating less inflammation. Overall 28 day post-MI survival rate was 68% with MI-N and 48% with MI-C. Importantly, there was no difference in infarct size or post-MI cardiac function between methods. This new rapid method of MI in mice represents a more efficient and less damaging model of myocardial ischemic injury compared to the classical method.