A method for in vitro fabrication of hybrid bone/cartilage tissue using mouse induced pluripotent stem cells
A method for in vitro fabrication of hybrid bone/cartilage tissue using mouse induced pluripotent stem cells
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一种利用小鼠诱导多能干细胞体外制造混合骨/软骨组织的方法
DOI：
10.1007/7651_2021_361
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Egusa H
中科院分区：
文献类型：
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作者：
Limraksasin P;Egusa H
在发育中的胚胎中，骨和软骨共享相同的祖细胞。然而，小鼠诱导多能干细胞（iPSC）的骨软骨诱导仍然很困难。在这里，我们描述了一个协议，以指导诱导多能干细胞分化成骨软骨细胞，形成混合骨/软骨结构在体外。首先将单个小鼠iPSC在超低附着微空间培养板中重新聚集。在第12天，使iPSC球经受振荡培养并在成骨诱导培养基中维持31天（Os诱导）。在另一种情况下，在第22天用软骨形成诱导培养基替换成骨诱导培养基，并再维持21天（Os-Chon诱导）。Os诱导产生了强烈的矿化和一些软骨样组织，而Os-Chon诱导导致部分矿化和大面积的软骨组织。
In the developing embryo, bone and cartilage share the same progenitors. However, osteo-chondrogenic induction of mouse induced pluripotent stem cells (iPSCs) remains difficult. Here we describe a protocol to guide iPSCs to differentiate into osteochondral cells that form hybrid bone/cartilage constructs in vitro. Single mouse iPSCs are first reaggregated in ultra-low-attachment micro-space culture plates. At day 12, iPSC spheres are subjected to shaking culture and maintained in an osteogenic induction medium for 31 days (Os induction). In another condition, the osteogenic induction medium is replaced by chondrogenic induction medium at day 22 and maintained for a further 21 days (Os-Chon induction). Os induction produced robust mineralization and some cartilage-like tissue, whereas Os-Chon induction resulted in partial mineralization and a large area of cartilage tissue.