Determination of nicotine and N-nitrosamines in house dust by pressurized liquid extraction and comprehensive gas chromatography-Nitrogen chemiluminiscence detection
Determination of nicotine and N-nitrosamines in house dust by pressurized liquid extraction and comprehensive gas chromatography-Nitrogen chemiluminiscence detection
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DOI：
10.1016/j.chroma.2011.11.017
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发表时间：
2012-01-06
影响因子：
4.1
通讯作者：
Hamilton, Jacqueline F.
中科院分区：
文献类型：
--
作者：
Ramirez, Noelia;Oezel, Mustafa Z.;Hamilton, Jacqueline F.
一种测定尼古丁的高选择性新方法。本文研究了室内灰尘样品中的N-亚硝胺和烟草特有的亚硝胺。样品通过单元内净化加压液体萃取（PLE）进行萃取，该萃取允许高萃取效率和适度的有机溶剂消耗。通过综合气相色谱法分析提取物，并使用氮荧光检测器（GC x GC-NCD）进行检测，该检测器对有机含氮化合物具有更高的选择性和灵敏度。方法学验证结果表明，线性关系良好，重复性和重现性良好（%RSD < 8%）。大多数目标化合物的回收率高于80%，检测限低于16 ng g（-1）。该方法被用于测定的亚硝胺目标化合物的室内灰尘样品从吸烟和不吸烟的家庭。所有的分析物都在样本中发现，尼古丁是吸烟者灰尘中最丰富的化合物，也是非吸烟者灰尘中最丰富的化合物之一。据我们所知，这是第一次，挥发性N-亚硝胺和TSNA已确定在室内灰尘样品。结果表明，这些高度致癌的化合物存在于室内灰尘，与固有的人类接触，通过吸入和/或非自愿摄入的室内灰尘。(C)2011 Elsevier B. V.保留所有权利。
A novel, highly selective method for the determination of nicotine. N-nitrosamines and tobacco-specific nitrosamines (TSNAs) in indoor dust samples is presented in this study. Samples were extracted by in-cell clean-up pressurized liquid extraction (PLE) that allows high extraction efficiency with moderate consumption of organic solvents. The extracts were analyzed by comprehensive gas chromatography and detected with a nitrogen chemiluminiscence detector (GC x GC-NCD) that provided enhanced selectivity and sensitivity for organic nitrogen containing compounds. Method validation showed good linearity, repeatability and reproducibility (%RSD < 8%). Recovery was higher than 80% for most target compounds and limits of detection lower than 16 ng g(-1). The method was used for the determination of the nitrosamine target compounds in house dust samples from both smoking and non-smoking households. All the analytes were found in the samples, nicotine being the most abundant compound in smokers' dust and one of the most abundant in non-smokers' dust. To our knowledge this is the first time that volatile N-nitrosamines and TSNAs have been determined in indoor dust samples. The results demonstrate the presence of these highly carcinogenic compounds in house dust, with inherent human exposure through inhalation and/or involuntary ingestion of house dust. (C) 2011 Elsevier B.V. All rights reserved.