Nanofat Grafting: Basic Research and Clinical Applications
Nanofat Grafting: Basic Research and Clinical Applications
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DOI：
10.1097/prs.0b013e31829fe1b0
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发表时间：
2013-10-01
影响因子：
3.6
通讯作者：
Declercq, Heidi
中科院分区：
文献类型：
--
作者：
Tonnard, Patrick;Verpaele, Alexis;Declercq, Heidi
背景：脂肪移植的适应症正在稳步增加。在微脂肪移植中，使用薄的注射套管。作者描述了他们的经验，脂肪注射更薄的注射针头高达27号。用于此目的的脂肪被加工成纳米脂肪。描述了临床应用。一项研究的初步结果，成立，以确定nanofat的细胞内容，presented.Methods：Nanofat移植进行了67例，以纠正浅表皱纹，疤痕，和黑暗的下眼睑。三个临床病例进行了描述。在这项研究中，分析了三个脂肪样本。第一个样本是典型的脂肪抽吸物（macrofat）。第二个样本是微脂肪，用多孔小孔套管采集。第三种是将微脂肪加工成纳米脂肪。处理包括脂肪抽吸物的乳化和过滤。分析脂肪样品的脂肪细胞活力。对来自间质血管组分和CD34（+）亚组分的细胞进行定量。通过在标准和成脂培养基中培养细胞来研究干细胞质量。结果：在纳米脂肪样品中没有观察到活的脂肪细胞。脂肪来源的干细胞仍然大量存在于纳米脂肪样品中。细胞培养物显示来自三个样品的干细胞的相等增殖和分化能力。临床应用表明，术后6个月皮肤质量有明显改善。没有感染，脂肪囊肿，肉芽肿，或其他不必要的副作用observed.Conclusions：纳米脂肪注射可能成为一个新的概念，在脂肪填充领域。在临床情况下，纳米脂肪似乎适合皮肤再生的目的。
Background: The indications for fat grafting are increasing steadily. In microfat grafting, thin injection cannulas are used. The authors describe their experience of fat injection with even thinner injection needles up to 27 gauge. The fat used for this purpose is processed into nanofat. Clinical applications are described. Preliminary results of a study, set up to determine the cellular contents of nanofat, are presented.Methods: Nanofat grafting was performed in 67 cases to correct superficial rhytides, scars, and dark lower eyelids. Three clinical cases are described. In the research study, three fat samples were analyzed. The first sample was a classic lipoaspirate (macrofat). The second sample was microfat, harvested with a multiport small-hole cannula. The third was microfat processed into nanofat. Processing consisted of emulsification and filtering of the lipoaspirate. Fat samples were analyzed for adipocyte viability. Cells from the stromal vascular fraction and the CD34(+) subfraction were quantified. The stem cell quality was investigated by culturing the cells in standard and adipogenic media.Results: No viable adipocytes were observed in the nanofat sample. Adipose-derived stem cells were still richly present in the nanofat sample. Cell cultures showed an equal proliferation and differentiation capacity of the stem cells from the three samples. Clinical applications showed remarkable improvements in skin quality 6 months postoperatively. No infections, fat cysts, granulomas, or other unwanted side effects were observed.Conclusions: Nanofat injections might become a new concept in the lipofilling area. In clinical situations, nanofat seems to be suitable for skin rejuvenation purposes.