Vitamin-D-dependent transcriptional regulation of the intestinal plasma membrane calcium pump.
Vitamin-D-dependent transcriptional regulation of the intestinal plasma membrane calcium pump.
复制标题
肠道质膜钙泵的维生素 D 依赖性转录调节。
DOI：
10.1006/bbrc.1995.2159
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Wasserman,RH
中科院分区：
文献类型：
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作者：
Pannabecker,TL;Chandler,JS;Wasserman,RH
维生素D激素1,25-二羟基维生素D3[1,25(OH)2D3]可增加肠质膜钙泵（PMCA）基因的表达。目前的研究是为了确定基因转录是否参与了这个过程。实验前不同时间心内注射1,25(OH) 2d3，从缺乏维生素d的雏鸡十二指肠分离细胞核。核糖核酸酶保护实验显示，在1.5小时时，细胞核和总细胞组分中PMCA RNA的丰度显著高于对照组。在3小时达到最大值。post-dose。如图所示，核PMCA RNA与细胞质RNA的交叉污染不能解释这些结果。这些研究首次表明，1,25(OH) 2d3通过增加转录速率来调节质膜钙泵基因的表达。
The vitamin D hormone, 1,25-dihydroxyvitamin D3[1,25(OH)2D3], was shown to increase intestinal plasma membrane calcium pump (PMCA) gene expression. The present study was done to determine whether gene transcription is involved in this process. Nuclei were isolated from duodena of vitamin-D-deficient chicks given 1,25(OH)2D3intracardially at various times before experiment. The abundance of PMCA RNA in the nuclear and total cellular fractions, measured by a ribonuclease protection assay, was significantly increased above control values at 1.5 hr. and maximized at 3 hr. post-dose. As shown, cross-contamination of nuclear PMCA RNA by cytosolic RNA cannot account for these results. These studies are the first to show that 1,25(OH)2D3regulates expression of a plasma membrane calcium pump gene by increasing the rate of transcription.