Development and evaluation of an efficient and real-time monitoring system for the vector mosquitoes, Aedes albopictus and Culex quinquefasciatus.
Development and evaluation of an efficient and real-time monitoring system for the vector mosquitoes, Aedes albopictus and Culex quinquefasciatus.
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DOI：
10.1371/journal.pntd.0010701
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发表时间：
2022-09
影响因子：
3.8
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中科院分区：
文献类型：
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蚊媒监测是预防和控制蚊媒疾病的关键。本研究开发了一种基于互联网的媒介蚊虫监测仪MS-300，并在实验室和现场试验中评价了其对重要媒介蚊虫白纹伊蚊和致倦库蚊的捕获效率。Ae. albopictus和Cx.并根据这些数据设计了红外窗口。开发了一种专门吸引这两种蚊子的设备，并将捕获的蚊子数量自动传输到互联网上。在实验室、半野外和开放野外试验中测试了该装置捕获这两种物种的效率。MS-300对Ae.对白纹伊蚊的杀灭率分别为98.5%和99.3%;对Cx的杀灭率分别为95.8%和98.6%。致倦肌在网筛房内进行半野外试验，研究了MS-300诱集Ae的效果。albopictus和Cx.对致倦库蚊的诱虫率分别为54.2%和51.3%，显著高于未诱虫的4%和4.2%。实时监测数据显示，Ae每天有两个活动高峰。白纹伊蚊（8：00-10：00和17：00-19：00），Cx有一个高峰。致倦鸡（20：00-24：00）。在为期98天的实地监测试验中，总共有1,118只Ae。albopictus和2，302 Cx.用MS-300捕捉致倦犬。捕获的蚊子数量与现场温度之间存在密切的相关性，并且使用MS-300、BioGents Sentinel诱捕器和人类登陆捕获的蚊子之间捕获样品的物种组成呈正相关。这些数据支持了MS-300可以特异性和有效地捕获Ae的结论。albopictus和Cx. quinquefasciatus，并实时自动监测其密度。因此，MS-300有可能用作预防和控制媒介蚊虫的监测工具。媒介蚊虫监测对预防和控制蚊媒疾病至关重要。然而，监测蚊子的传统方法可能不符合道德要求，而且是劳动密集型的。我们开发了一种基于互联网的媒介蚊子监测器MS-300，它可以灵敏地检测蚊子进入并自动将数据传输到基于云的服务器服务和远程终端。通过实验室、半现场和现场试验，评价了该系统对重要媒介蚊虫白纹伊蚊和致倦库蚊的捕捉效果。结果表明，MS-300装置可用于Ae的监测。albopictus和Cx. quinquefasciatus的结果与人类登陆渔获量和BioGents哨兵陷阱。MS-300监测仪是评估Ae的可靠工具。albopictus和Cx.在实验室和现场调查中，可以利用致倦犬种群密度和昼夜活动模式，并在应用于每日和季节性种群波动时，用于指导蚊媒疾病的预防和控制工作。
The surveillance of vector mosquitoes is essential for prevention and control of mosquito-borne diseases. In this study, we developed an internet-based vector mosquito monitor, MS-300, and evaluated its efficiency for the capture of the important vector mosquitoes, Aedes albopictus and Culex quinquefasciatus, in laboratory and field trials. The linear sizes of adult Ae. albopictus and Cx. quinquefasciatus were measured and an infrared window was designed based on these data. A device to specifically attract these two species and automatically transmit the number of captured mosquitoes to the internet was developed. The efficiency of the device in capturing the two species was tested in laboratory, semi-field and open field trials. The efficiency results for MS-300 for catching and identifying Ae. albopictus in laboratory mosquito-net cages were 98.5% and 99.3%, and 95.8% and 98.6%, respectively, for Cx. quinquefasciatus. In a wire-gauze screened house in semi-field trials, the efficiencies of MS-300 baited with a lure in catching Ae. albopictus and Cx. quinquefasciatus were 54.2% and 51.3%, respectively, which were significantly higher than 4% and 4.2% without the lure. The real-time monitoring data revealed two daily activity peaks for Ae. albopictus (8:00–10:00 and 17:00–19:00), and one peak for Cx. quinquefasciatus (20:00–24:00). During a 98-day surveillance trial in the field, totals of 1,118 Ae. albopictus and 2,302 Cx. quinquefasciatus were captured by MS-300. There is a close correlation between the number of captured mosquitoes and the temperature in the field, and a positive correlation in the species composition of the captured samples among the mosquitoes using MS-300, BioGents Sentinel traps and human landing catches. The data support the conclusion that MS-300 can specifically and efficiently capture Ae. albopictus and Cx. quinquefasciatus, and monitor their density automatically in real-time. Therefore, MS-300 has potential for use as a surveillance tool for prevention and control of vector mosquitoes. Surveillance of vector mosquitoes is essential for prevention and control of mosquito-borne diseases. However, traditional methods for monitoring mosquitoes may not meet ethical requirements and are labor-intensive. We developed an internet-based vector mosquito monitor, MS-300, that can sensitively detect mosquito entry and automatically transmit the data to cloud-based server services and remote terminals. We evaluated its efficiency for the capture of the important vector mosquitoes, Aedes albopictus and Culex quinquefasciatus, in laboratory, semi-field and field trials. Our results demonstrate that the MS-300 device could monitor Ae. albopictus and Cx. quinquefasciatus with results comparable with human landing catches and BioGents Sentinel traps. The MS-300 monitor can be a dependable tool for assessing Ae. albopictus and Cx. quinquefasciatus population densities and circadian activity patterns in both laboratory and field surveys and can be used to guide prevention and control efforts of mosquito-borne diseases when applied to daily and seasonal population fluctuations.
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