CELLULAR FACTORS REQUIRED FOR MULTIPLE STAGES OF SV40 DNA-REPLICATION INVITRO
CELLULAR FACTORS REQUIRED FOR MULTIPLE STAGES OF SV40 DNA-REPLICATION INVITRO
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DOI：
10.1002/j.1460-2075.1988.tb02933.x
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发表时间：
1988-04-01
期刊：
影响因子：
11.4
通讯作者：
STILLMAN, B
中科院分区：
文献类型：
--
作者：
FAIRMAN, MP;STILLMAN, B
含有SV40起源的质粒会在SV40T抗原和来自人293细胞的无细胞提取物的存在下复制。对这种提取物进行分级后，鉴定出两个重要的复制因子。其中一种是含有70,000，34,000和11,000道尔顿多肽的多亚基DNA结合蛋白，其功能可能是真核单链DNA结合蛋白(SSB)。另一个部分纯化的部分与T抗原一起用于DNA复制的第一阶段，即在复制起始处形成预合成复合体。这些结果和其他结果定义了SV40在体外复制的多个阶段，类似于大肠杆菌和噬菌体的多个阶段。复制，并可能反映类似的事件在细胞染色体的复制。
Plasmids containing the SV40 origin replicate in the presence of SV40 T antigen and a cell free extract derived from human 293 cells. Upon fractionation of this extract, two essential replication factors have been identified. One of these is a multi-subunit DNA binding protein containing polypeptides of 70,000, 34,000 and 11,000 daltons which may function as a eukaryotic single strand DNA binding protein (SSB). The other partially purified fraction is required with T antigen for the first stage of DNA replication, the formation of a pre-synthesis complex at the replication origin. These results, and others, define multiple stages of SV40 replication in vitro which are analogous to multiple stages of Escherichia coli and phage .lambda. replication, and may reflect similar events in the replication of cellular chromosomes.