miR-21 targets the tumor suppressor RhoB and regulates proliferation, invasion and apoptosis in colorectal cancer cells
miR-21 targets the tumor suppressor RhoB and regulates proliferation, invasion and apoptosis in colorectal cancer cells
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miR-21靶向肿瘤抑制因子RhoB并调节结直肠癌细胞的增殖、侵袭和凋亡
DOI：
10.1016/j.febslet.2011.08.014
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发表时间：
2011-10-03
期刊：
影响因子：
3.5
通讯作者：
Bi, Feng
中科院分区：
文献类型：
--
作者：
Liu, Ming;Tang, Qinlin;Bi, Feng
越来越清楚的是，microRNA在包括癌症在内的许多人类疾病中起着重要作用。在此，我们发现miR-21在HEK 293和几种结直肠癌细胞中的表达与ras同源基因家族成员B（Rho B）的表达呈负相关。miR-21的表达显著抑制RhoB 3' UTR报告酶活性，但当假定的靶位点突变时，抑制作用丧失。在结直肠癌细胞中，外源性miR-21过表达模拟RhoB敲低在促进增殖和侵袭以及抑制凋亡方面的作用，而抗miR-21或RhoB表达产生相反的作用。这些结果表明，miR-21是RhoB表达的调节因子，RhoB可能是探索miR-21介导的结直肠癌肿瘤细胞行为的潜在治疗益处的有用靶点。(C)2011年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
It has become increasingly clear that microRNAs play an important role in many human diseases including cancer. Here, we show that expression of miR-21 in HEK293 and several colorectal cancer cells was found inversely correlated with ras homolog gene family, member B (RhoB) expression. miR-21 expression significantly suppressed RhoB 3' UTR luciferase-reporter activity, but the inhibitory effect was lost when the putative target sites were mutated. Exogenous miR-21 over-expression mimicked the effect of RhoB knockdown in promoting proliferation and invasion and inhibiting apoptosis, whereas anti-miR-21 or RhoB expression yielded opposite effects, in colorectal cancer cells. These results suggest that miR-21 is a regulator of RhoB expression and RhoB could be a useful target in exploring the potential therapeutic benefits of miR-21 mediated tumor cell behaviors in colorectal cancer. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.