Electrochemiluminescence immunosensor based on graphene-CdS quantum dots-agarose composite for the ultrasensitive detection of alpha fetoprotein.
Electrochemiluminescence immunosensor based on graphene-CdS quantum dots-agarose composite for the ultrasensitive detection of alpha fetoprotein.
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DOI：
10.1016/j.talanta.2011.11.017
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发表时间：
2012-01
期刊：
影响因子：
6.1
通讯作者：
Zhiyong Guo;Tingting Hao;Jing Duan;Sui Wang;Danyi Wei
中科院分区：
文献类型：
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作者：
Zhiyong Guo;Tingting Hao;Jing Duan;Sui Wang;Danyi Wei
将CdS量子点（QDs）、石墨烯（G）和琼脂糖（Agarose）结合起来，提出了一种新的电化学发光增强策略。这种增强的ECL被用来开发一种用于超灵敏检测甲胎蛋白（AFP）的无标记ECL免疫传感器。首次将G-CdS量子点-琼脂糖复合物涂覆在玻碳电极表面形成一层牢固的薄膜，该薄膜具有高的电化学发光强度、良好的生物相容性和高的稳定性。将3-氨丙基三乙氧基硅烷（APS）作为结合剂与G-CdS量子点-琼脂糖复合膜偶联后，电化学发光信号明显增强。通过戊二醛（GLD）将甲胎蛋白抗体（Ab）固定在电极上，成功地制备了ECL免疫传感器。甲胎蛋白与抗体发生特异性免疫反应，导致电化学发光强度降低，在0.0005- 50 pgmL-1范围内，电化学发光强度与甲胎蛋白浓度的对数呈线性关系，检测限为0.2fgmL-1。该免疫传感器具有较高的灵敏度、特异性、稳定性、重现性和良好的再生性，具有应用于临床的潜力。此外，G-CdS量子点-琼脂糖复合膜的电化学发光的高度增强为石墨烯和量子点电化学发光在分析系统和电化学发光生物传感器中的应用开辟了新的途径。
A novel strategy for the enhancement of electrochemiluminescence (ECL) was developed by combining CdS quantum dots (QDs), graphene (G) and agarose. This enhanced ECL was exploited to develop a label-free ECL immunosensor for the ultrasensitive detection of alpha fetoprotein (AFP). The novel G–CdS QDs–agarose composite was first coated on the glass carbon electrode surface to form a robust film, which exhibited high ECL intensity, good biocompatibility and high stability. After that 3-aminopropyl-triethoxysilane (APS), as a binding linker, was conjugated to the G–CdS QDs–agarose composite film on the electrode, the ECL signal was significantly enhanced. The fabrication of ECL immunosensor was successfully completed by immobilizing the AFP-antibody (Ab) onto the electrode through glutaric dialdehyde (GLD). The specific immunoreaction between AFP and antibody resulted in the decrease in ECL intensity and the intensity decreased linearly with the logarithm of AFP concentration in the range of 0.0005–50pgmL−1with a detection limit of 0.2fgmL−1. The immunosensor exhibits high sensitivity, specificity, stability, reproducibility and good regeneration, thus has the potential to be used in clinical application. Besides, the highly enhanced ECL from the G–CdS QDs–agarose composite film opened new avenues to apply graphene and QDs ECL in analytical systems and ECL biosensors.