Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation
Intracranial self-stimulation (ICSS) in rodents to study the neurobiology of motivation
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DOI：
10.1038/nprot.2007.441
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Chartoff, Elena H.
中科院分区：
文献类型：
--
作者：
Carlezon, William A., Jr.;Chartoff, Elena H.
评估实验室动物的情绪状态变得越来越重要。反映奖励、体验奖励的能力降低（快感缺乏）和厌恶（烦躁不安）的测试需求很高，因为许多精神疾病目前在人类中难以治愈（例如，例如，在一个实施例中，严重抑郁症、双相情感障碍、成瘾）的特征是动机失调。颅内自我刺激（ICSS）可用于啮齿动物（大鼠，小鼠），以了解药理学或分子操作如何影响大脑奖励系统的功能。虽然许多不同的方法是可能的，我们将在本协议中描述使用内侧前脑束（MFB）刺激与“曲线移位”分析的变体。这种组合是特别强大的，因为它产生了一个高度可靠的行为输出，使动机的治疗效果和执行任务的能力的治疗效果之间的明确区分。
It has become increasingly important to assess mood states in laboratory animals. Tests that reflect reward, reduced ability to experience reward ( anhedonia) and aversion ( dysphoria) are in high demand because many psychiatric conditions that are currently intractable in humans ( e. g., major depression, bipolar disorder, addiction) are characterized by dysregulated motivation. Intracranial self-stimulation (ICSS) can be utilized in rodents ( rats, mice) to understand how pharmacological or molecular manipulations affect the function of brain reward systems. Although many different methodologies are possible, we will describe in this protocol the use of medial forebrain bundle (MFB) stimulation together with the 'curve-shift' variant of analysis. This combination is particularly powerful because it produces a highly reliable behavioral output that enables clear distinctions between the treatment effects on motivation and the treatment effects on the capability to perform the task.