Sorghum Root Flavonoid Chemistry, Cultivar, and Frost Stress Effects on Rhizosphere Bacteria and Fungi
Sorghum Root Flavonoid Chemistry, Cultivar, and Frost Stress Effects on Rhizosphere Bacteria and Fungi
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DOI：
10.1094/pbiomes-01-20-0013-fi
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发表时间：
2021-01-01
影响因子：
4.4
通讯作者：
Chopra, Surinder
中科院分区：
文献类型：
--
作者：
Cloutier, Mara;Chatterjee, Debamalya;Chopra, Surinder
包括真菌感染在内的生物胁迫会导致高粱叶组织中黄酮类化合物的产量增加，其中包括 3-脱氧花青素 (3-DA)。我们的目标是确定高粱基因型变异是否影响根类黄酮和 3-DA 浓度以及根际微生物群落，并确定这些关系如何受到非生物胁迫的影响。我们评估了晚霜霜前后五个近等基因高粱品系的根部化学物质和根际微生物组。分析根部的总黄酮类、总酚类、3-DA 浓度和抗氧化活性。在根际土壤上进行 16S 核糖体 RNA 基因和内部转录间隔区的扩增子测序。霜冻前，几个品系中木犀草素（3-DA）和总黄酮的浓度存在差异；然而，这些关系在霜冻之后发生了变化。霜后，三个品系的木犀草素含量增加，而霜后所有品系的总黄酮含量下降。霜前和霜后木犀草素和总类黄酮浓度不同的品系也不同。霜冻前后根际群落组成也有所不同，但高粱品系之间只有真菌群落组成不同。细菌群落组成与总黄酮和木犀草素浓度高度相关。此外，与真菌类群相比，更多的细菌类群与总黄酮类化合物和木犀草素相关。总的来说，这项研究提供了证据，表明植物基因型变异影响根类黄酮和沟圈群落组成，并且这些关系受到霜冻的影响。植物-微生物相互作用和次生代谢产物的产生可能是高粱耐霜冻选择性育种的重要组成部分。
Biotic stresses, including fungal infections, result in increased production of flavonoid compounds, including 3-deoxyanthocyanidins (3-DAs), in the leaf tissues of Sorghum bicolor. Our objectives were to determine whether sorghum genotypic variation influenced root flavonoid and 3-DA concentrations and rhizosphere microbial communities and to identify how these relationships were affected by abiotic stress. We evaluated root chemicals and rhizosphere microbiomes of five near-isogenic lines of sorghum before and after a late-season frost. Roots were analyzed for total flavonoids, total phenolics, 3-DA concentrations, and antioxidant activity. Amplicon sequencing of 16S ribosomal RNA genes and internal transcribed spacer regions was performed on rhizosphere soils. Concentrations of luteolinidin (a 3-DA) and total flavonoids differed between several lines before frost; however, these relationships changed after frost. Luteolinidin increased in three lines after frost, whereas total flavonoids decreased in all the lines after frost. Lines that differed in luteolinidin and total flavonoid concentrations before frost were different from those after frost. Rhizosphere community compositions also differed before and after frost but only fungal community compositions differed among sorghum lines. Bacterial community compositions were highly correlated with total flavonoid and luteolinidin concentrations.Furthermore, a greater number of bacterial taxa were correlated with total flavonoids and luteolinidin compared with fungal taxa. Collectively, this study provides evidence that plant genotypic variation influences root flavonoids and Mizosphere community composition and that these relationships are affected by frost. Plant-microbe interactions and secondary metabolite production may be important components to include for selective breeding of sorghum for frost stress tolerance.