Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat
Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat
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开发用于普通小麦野生近缘种 Haynaldia villosa 的 FISH 核型分析寡核苷酸探针
DOI：
10.1016/j.cj.2020.02.008
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发表时间：
2020-08-01
期刊：
影响因子：
6.6
通讯作者：
Wang, Xiue
中科院分区：
文献类型：
--
作者：
Lei, Jia;Zhou, Jiawen;Wang, Xiue
长绒海纳藻是小麦的野生亲缘植物，是小麦改良的宝贵基因资源。由于可用于荧光原位杂交（FISH）的探针数量有限，绒毛猴核型表征的分辨率很差，阻碍了对小片段外来基因渗入的准确表征。我们设计了10个寡核苷酸探针，采用串联重复序列的DNA序列衍生自长绒猴染色体6V （6VS）短臂。其中7个的FISH在长绒棉染色体上产生了清晰的信号。在此基础上，我们利用oligo-6VS-1和oligo-6VS-35寡核苷酸构建了绒毛猪的FISH核型，并对这两种探针在5个不同绒毛猪种群中的分布进行了表征。新的FISH探针可以有效地表征稻瘟病菌在小麦中的渗入情况。(C) 2020年中国作物科学学会，中国农业科学院作物科学研究所；由Elsevier B.V.代表KeAi Communications Co. Ltd制作和主持。
Haynaldia villosa is a wild relative of wheat and a valuable gene resource for wheat improvement. Owing to the limited number of probes available for fluorescence in situ hybridization (FISH), the resolution at which the karyotype of H. villosa can be characterized is poor, hampering accurate characterization of small segmental alien introgressions. We designed ten oligonucleotide probes using tandem repeats in DNA sequences derived from the short arm of H. villosa chromosome 6V (6VS). FISH with seven of them resulted in clear signals on H. villosa chromosomes. Using these, we constructed FISH karyotypes for H. villosa using oligo-6VS-1 and oligo-6VS-35 oligonucleotides and characterized the distribution of the two probes in five different H. villosa accessions. The new FISH probes can efficiently characterize H. villosa introgressions into wheat. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.