Transcriptome profiling in fast versus slow-growing rainbow trout across seasonal gradients.
Transcriptome profiling in fast versus slow-growing rainbow trout across seasonal gradients.
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DOI：
10.1186/s12864-016-2363-5
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发表时间：
2016-01-15
期刊：
影响因子：
4.4
通讯作者：
Palti Y
中科院分区：
文献类型：
--
作者：
Danzmann RG;Kocmarek AL;Norman JD;Rexroad CE 3rd;Palti Y
脊椎动物的周年节律可以影响各种各样的生理过程。一些值得注意的例子包括年度生殖周期，对于变温动物来说，季节性变化调节着生长。水温升高会提高鱼类的生长速度，但即使在恒温条件下，光周期的增加也会产生类似的影响。因此，为了了解鱼类的生长动态，考虑光周期对基因表达差异的背景影响是很重要的。本研究从商业水产养殖品系出发，比较了递减的光周期(冬至)和递增的光周期(春分)对快速和缓慢生长彩虹鲑白肌肉转录组的影响。生长缓慢的鱼可以被描述为拥有在许多方面符合人类耐力训练制度的转录组特征。它们提高了线粒体和胞浆肌酸激酶的表达水平，并似乎抑制了mTOR信号转导，这一点从TSC2的表达增加可见一斑，它们还具有升高的P53水平。大型鱼类表现出一种可能与力量/抗性生理学相一致的生理过程，细胞骨架基因组分的表达和糖原代谢循环伴随着更高的PI3K水平。在许多方面，小鱼和大鱼分别与偏心和同心的肌肉表达模式相匹配。在体型较大的鱼类中，脂肪代谢基因也更高，最值得注意的是G0S2开关基因。M型和Z型肌节重塑似乎在大型鱼类中更为普遍。在之前报道的26个基于SNP的生长差异显著的基因家族中，有23个基因家族被检测到具有显著的表达差异。较大的鱼表现出更广泛的基因阵列，显示出更高的表达，它们的图谱与12月份观察到的批次鱼(即加速生长期)更相似。相反，小型鱼显示的基因图谱更类似于季节性生长衰退阶段(即9月底的鱼)。总体而言，与鱼类大小相关的差异相比，季节性时机与基因表达的差异更大。本文的在线版本(doi：10.1186/s12864-0162363-5)包含补充材料，可供授权用户使用。
Circannual rhythms in vertebrates can influence a wide variety of physiological processes. Some notable examples include annual reproductive cycles and for poikilotherms, seasonal changes modulating growth. Increasing water temperature elevates growth rates in fishes, but increases in photoperiod regime can have similar influences even at constant temperature. Therefore, in order to understand the dynamics of growth in fish it is important to consider the background influence of photoperiod regime on gene expression differences. This study examined the influence of a declining photoperiod regime (winter solstice) compared to an increasing photoperiod regime (spring equinox) on white muscle transcriptome profiles in fast and slow-growing rainbow trout from a commercial aquaculture strain. Slow-growing fish could be characterized as possessing transcriptome profiles that conform in many respects to an endurance training regime in humans. They have elevated mitochondrial and cytosolic creatine kinase expression levels and appear to suppress mTOR-signaling as evidenced by elevated TSC2 expression, and they also have elevated p53 levels. Large fish display a physiological repertoire that may be consistent with strength/resistance physiology having elevated cytoskeletal gene component expression and glycogen metabolism cycling along with higher PI3K levels. In many respects small vs. large fish match eccentric vs. concentric muscle expression patterns, respectively. Lipid metabolic genes are also more elevated in larger fish, the most notable being the G0S2 switch gene. M and Z-line sarcomere remodelling appears to be more prevalent in large fish. Twenty-three out of 26 gene families with previously reported significant SNP-based growth differences were detected as having significant expression differences. Larger fish display a broader array of genes showing higher expression, and their profiles are more similar to those observed in December lot fish (i.e., an accelerated growth period). Conversely, small fish display gene profiles more similar to seasonal growth decline phases (i.e., September lot fish). Overall, seasonal timing was coupled to greater differences in gene expression compared to differences associated with fish size. The online version of this article (doi:10.1186/s12864-016-2363-5) contains supplementary material, which is available to authorized users.