Mapping of Sheep Sensory Cortex With a Novel Microelectrocorticography Grid
Mapping of Sheep Sensory Cortex With a Novel Microelectrocorticography Grid
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DOI：
10.1002/cne.23631
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发表时间：
2014-11-01
影响因子：
2.5
通讯作者：
Ball, Tonio
中科院分区：
文献类型：
--
作者：
Gierthmuehlen, Mortimer;Wang, Xi;Ball, Tonio
显微皮质电图（mu ECoG）提供了对皮层组织的高时间和空间分辨率的见解，有助于更好地理解神经信息处理。在这里，我们评估了在羊模型中使用mu ECoG对躯体感觉诱发电位（sep）进行详细的皮质记录。该方法是利用基于核磁共振成像的绵羊大脑3D模型来计划的。我们描述了一种最小扩展的外科手术程序，允许在体感觉皮层上放置两个不同的mu ECoG网格。通过这种小开颅术，额窦保持完整，从而保持手术部位无菌，使该入路适合慢性植入。我们评估了慢性植入胶囊化脑电图记录系统的方法。在急性和慢性记录中，在所有动物中都发现了在鞍状沟、对角线沟和冠状沟之间的三角形有明显的SEP反应。对鼻子、上唇、下唇和下巴的刺激可引起体位侧向内、同侧的反应模式。通过重复记录SEPs，这种体位模式被可靠地记录了长达16周。本研究的发现证实了先前假设的羊感觉皮层的同侧体位组织。在体感觉诱发反应的不同频率成分的比较中，高伽马波段的活动在空间上最具特异性。本研究通过描述羊的感觉皮层的确切位置、功能特性以及与宏观解剖标志相关的手术入路，为进一步的急性和慢性调查提供了基础。中国生物医学工程学报，2014,22(2):379 - 379。(c) 2014 Wiley期刊公司
Microelectrocorticography (mu ECoG) provides insights into the cortical organization with high temporal and spatial resolution desirable for better understanding of neural information processing. Here we evaluated the use of mu ECoG for detailed cortical recording of somatosensory evoked potentials (SEPs) in an ovine model. The approach to the cortex was planned using an MRI-based 3D model of the sheep's brain. We describe a minimally extended surgical procedure allowing placement of two different mu ECoG grids on the somatosensory cortex. With this small craniotomy, the frontal sinus was kept intact, thus keeping the surgical site sterile and making this approach suitable for chronic implantations. We evaluated the procedure for chronic implantation of an encapsulated mu ECoG recording system. During acute and chronic recordings, significant SEP responses in the triangle between the ansate, diagonal, and coronal sulcus were identified in all animals. Stimulation of the nose, upper lip, lower lip, and chin caused a somatotopic lateral-to-medial, ipsilateral response pattern. With repetitive recordings of SEPs, this somatotopic pattern was reliably recorded for up to 16 weeks. The findings of this study confirm the previously postulated ipsilateral, somatotopic organization of the sheep's sensory cortex. High gamma band activity was spatially most specific in the comparison of different frequency components of the somatosensory evoked response. This study provides a basis for further acute and chronic investigations of the sheep's sensory cortex by characterizing its exact position, its functional properties, and the surgical approach with respect to macroanatomical landmarks. J. Comp. Neurol. 522:3590-3608, 2014. (c) 2014 Wiley Periodicals, Inc.