Isolation and enumeration of marine actinomycetes from seawater and sediments in Alexandria
Isolation and enumeration of marine actinomycetes from seawater and sediments in Alexandria
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DOI：
10.2323/jgam.46.105
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发表时间：
2000-06-01
影响因子：
1.2
通讯作者：
Abu El-Ela, GA
中科院分区：
文献类型：
--
作者：
Ghanem, NB;Sabry, SA;Abu El-Ela, GA
从亚历山大港4个不同地点的近岸海洋沉积物和水中分离到放线菌，统计分析表明，温度、pH和溶解性磷的变化不显著，但总氮和有机质的变化显著，沉积物和水样经加热处理后，非放线菌异养微生物区系选择性减少。硝酸盐淀粉培养基有利于这些微生物的生长。放线菌的多样性和数量随季节变化而变化，干暖季节放线菌数量最高，沉积物中放线菌数量远远超过海水中放线菌的数量，种群数量与地理位置呈正相关。放线菌数量最多的是上层(0~20 cm)。在少数情况下，放线菌的数量呈现出0-20和60-100 cm深的双峰，沉积物是海洋放线菌的最佳来源，其分布随采样深度的不同而不同，放线菌占总微生物区系的比例在0.48-2.29之间，取决于位置。
Actinomycetes were isolated from near-shore marine sediments and water at four different sites in Alexandria, Statistical analysis revealed that variation in temperature, pH, and dissolved phosphate were of insignificant values, but that variation in total nitrogen and organic matter were significant, The treatment of sediments and water samples by heat resulted in a selective reduction of the nonactinomycetal heterotrophic microflora, Four selective culture media were used for counting actinomycetes in marine water and sediments. The starch nitrate medium favored the growth of these microorganisms. The diversity and counts of actinomycetes varied with the seasonal variation, and the highest counts were detected in dry warm seasons, The numbers of this bacterial group in sediments exceeded by far their numbers in seawater, A positive correlation was found between population size and location. Actinomycetes were found in the highest numbers in the upper layers (0-20cm depth). In a few cases, the counts of actinomycetes showed bimodal maxima 0-20 and 60-100cm deep, Sediments were the best source of marine actinomycetes, and their distribution varied depending on the depth from which samples were collected, The ratio of actinomycetes to the total microflora ranged from 0.48 to 2.29, depending on location.