MB78, A VIRULENT BACTERIOPHAGE OF SALMONELLA-TYPHIMURIUM
MB78, A VIRULENT BACTERIOPHAGE OF SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jvi.41.3.1038-1043.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
CHAKRAVORTY, M
中科院分区：
文献类型：
--
作者：
JOSHI, A;SIDDIQI, JZ;CHAKRAVORTY, M
本文报道了一株与P22在形态、血清学和生理上均不相关的鼠伤寒沙门氏菌（S. typhimurium）强毒噬菌体MB78的分离及其部分特性。噬菌体有一条不可收缩的长尾巴，尾巴的两端是分节的。它不能在柠檬酸盐存在的最小培养基中繁殖。感染mb78的细胞在这种培养基中被杀死。这种噬菌体不能在宿主的抗利福平突变体中生长。该噬菌体的生长潜伏期比P22短得多。为了排除重复感染的MB78噬菌体，需要驻留P22噬菌体的sieA和sieB基因；所有与P22相关的温带噬菌体都被1个或两个基因单独排除。P22和MB78的限制性内切酶切割模式明显不同。2个噬菌体P22和MB78之间没有同源性，表明MB78与噬菌体P22无关。
The isolation and some properties of a virulent bacteriophage of S. typhimurium, MB78, which is morphologically, serologically and physiologically unrelated to P22, are reported. The phage has a noncontractile long tail with partite ends. It cannot multiply in minimal medium in the presence of citrate. MB78-infected cells are killed in such medium. This phage cannot grow in rifampin-resistant mutants of the host. The latent period of growth of this phage is much shorter than that of P22. Both sieA and sieB genes of the resident P22 prophage are required to exclude the superinfecting MB78 phage; all temperate phages related to P22 are excluded by either 1 or both of the genes individually. Restriction endonuclease cleavage patterns of P22 and MB78 are distinctly different. The absence of homology between the 2 phages P22 and MB78 suggests that MB78 is not related to phage P22.