CEA-related cell adhesion molecule 1:: A potent angiogenic factor and a major effector of vascular endothelial growth factor
CEA-related cell adhesion molecule 1:: A potent angiogenic factor and a major effector of vascular endothelial growth factor
复制标题
DOI：
10.1016/s1097-2765(00)80426-8
复制
发表时间：
2000-02-01
期刊：
影响因子：
16
通讯作者：
Wagener, C
中科院分区：
文献类型：
--
作者：
Ergün, S;Kilic, N;Wagener, C
在体外和体内血管生成实验中，cpa相关细胞粘附分子1 （CEACAM1）显示出血管生成特性。从粒细胞和内皮细胞培养基中纯化的CEACAM1以及在HEK293细胞中表达的重组CEACAM1可刺激人微血管内皮细胞的增殖、趋化性和毛细血管样管的形成。它们增加了鸡绒毛膜尿囊膜的血管化，增强了血管内皮生长因子（VEGF）的作用（165）。VEGF（165）在mRNA和蛋白水平上均增加CEACAM1的表达。单克隆CEACAM1抗体可阻断VEGF（165）诱导的内皮管形成。这些数据表明CEACAM1在早期微血管形成中是VEGF的主要效应因子。由于CEACAM1在肿瘤微血管中表达，而不在大血管中表达，因此CEACAM1可能是抑制肿瘤血管生成的靶点。
CPA-related cell adhesion molecule 1 (CEACAM1) exhibits angiogenic properties in in vitro and in vivo angiogenesis assays. CEACAM1 purified from granulocytes and endothelial cell media as well as recombinant CEACAM1 expressed in HEK293 cells stimulate proliferation, chemotaxis, and capillary-like tube formation of human microvascular endothelial cells. They increase vascularization of chick chorioallantoic membrane and potentiate the effects of vascular endothelial growth factor (VEGF)(165). VEGF(165) increases CEACAM1 expression both on the mRNA and the protein level. VEGF(165)-induced endothelial tube formation is blocked by a monoclonal CEACAM1 antibody. These data suggest that CEACAM1 is a major effector of VEGF in the early microvessel formation. Since CEACAM1 is expressed in tumor microvessels but not in large blood vessels, CEACAM1 may be a target for the inhibition of tumor angiogenesis.