Fabrication consideration of three-dimensional skeletal muscle fiber using biomaterial
Fabrication consideration of three-dimensional skeletal muscle fiber using biomaterial
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DOI：
10.1109/mhs.2017.8305208
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Yuta Iwamoto;K. Ohara;Masaru Takeuchi;A. Ichikawa;T. Fukuda
中科院分区：
文献类型：
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作者：
Yuta Iwamoto;K. Ohara;Masaru Takeuchi;A. Ichikawa;T. Fukuda
本文提出了利用生物材料、C2C12细胞和微流控装置制备人工肌束的方法。将含C2C12细胞的凝胶纤维为基础的纤维蛋白进行整合，实现了模拟天然肌束的人工肌束。在培养第1天的荧光染色中，合成的集成凝胶纤维显示出高的细胞活力和高的各向异性细胞伸长。因此，在不久的将来，长期的细胞培养和通过分化获得肌肉收缩能力有望实现。
In this paper, we proposed fabrication method of artificial muscle bundle using biomaterial, C2C12 cells and microfluidic device. Artificial muscle bundle aiming at mimicking of nature muscle bundle was accomplished by integration of gel fiber based fibrin containing C2C12 cells. Fabricated integrated gel fiber was demonstrated high cell viability and high anisotropic cell elongation at 1 day of culture from fluorescent staining. As a result, long-tern cell culture and acquisition of muscle contraction ability by differentiation are expected in the near future.