Exercise-stimulated glucose transport in skeletal muscle is nitric oxide dependent
Exercise-stimulated glucose transport in skeletal muscle is nitric oxide dependent
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DOI：
10.1152/ajpendo.1997.273.1.e220
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发表时间：
1997-07-01
影响因子：
5.1
通讯作者：
Balon, TW
中科院分区：
文献类型：
--
作者：
Roberts, CK;Barnard, RJ;Balon, TW
有研究表明，骨骼肌中存在胰岛素刺激和收缩刺激的葡萄糖转运途径。本研究通过分离的肌膜制剂和一氧化氮合酶抑制剂n -omega-硝基- l -精氨酸甲酯（L-NAME），在饮用水中（1mg /ml）检测了一氧化氮对大鼠骨骼肌葡萄糖运输的影响。雌性Sprague-Dawley大鼠分为对照组、急性运动组、急性运动+ L-NAME组、胰岛素刺激组和胰岛素刺激+ L-NAME组。运动（45分钟穷尽式跑步机）增加葡萄糖转运（37 +/- 2至76 +/- 5 pmol.mg）。15 s(-1)), L-NAME （40 +/- 4 pmol.mg(-1)）完全抑制这种增加。15秒(1))。胰岛素的最大剂量增加葡萄糖转运（87 +/- 10 pmol.mg）。15 s(-1))，添加L-NAME （87 +/- 11 pmol.mg(-1)）无影响。15秒(1))。此外，运动，而不是运动+ L-NAME，增加了肌膜GLUT-4含量。这项研究证实，收缩和胰岛素刺激的葡萄糖运输有不同的途径。更重要的是，尽管运动和胰岛素都显著增加了葡萄糖的转运，但L-NAME对胰岛素刺激的葡萄糖转运没有影响，反而阻断了运动刺激的转运。我们得出的结论是，一氧化氮参与了运动过程中葡萄糖转运的信号转导机制。
It has been suggested that there are separate insulin-stimulated and contraction-stimulated glucose transport pathways in skeletal muscle. This study examined the effects of nitric oxide on glucose transport in rat skeletal muscle by use of an isolated sarcolemmal membrane preparation and the nitric oxide synthase inhibitor N-omega-nitro-L-arginine methyl ester (L-NAME), administered in the drinking water (1 mg/ml). Female Sprague-Dawley rats were divided into five groups: control, acute exercise, acute exercise + L-NAME, insulin stimulated, and insulin stimulated + L-NAME. Exercise (45 min of exhaustive treadmill running) increased glucose transport (37 +/- 2 to 76 +/- 5 pmol.mg(-1).15 s(-1)), and this increase was completely inhibited by L-NAME (40 +/- 4 pmol.mg(-1).15 s(-1)). A maximum dose of insulin increased glucose transport (87 +/- 10 pmol.mg(-1).15 s(-1)), and adding L-NAME had no effect (87 +/- 11 pmol.mg(-1).15 s(-1)). In addition, exercise, but not exercise + L-NAME, increased sarcolemma GLUT-4 content. This study confirms that there are separate pathways for contraction- and insulin-stimulated glucose transport. More importantly, although exercise and insulin both significantly increased glucose transport, L-NAME had no effect on insulin-stimulated glucose transport but blocked the exercise-stimulated transport. We conclude that nitric oxide is involved in the signal transduction mechanism to increase glucose transport during exercise.