Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilisation and/or electrical activation
Pronuclear formation and intracellular glutathione content of in vitro-matured porcine oocytes following in vitro fertilisation and/or electrical activation
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体外受精和/或电激活后体外成熟猪卵母细胞的原核形成和细胞内谷胱甘肽含量
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Billy N. Day
中科院分区：
文献类型：
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作者：
Hiroaki Funahashi;T. Stumpf;T. Cantley;N. Kim;Billy N. Day
在授精和/或电刺激后，测定了体外成熟的猪卵母细胞中原核形成和细胞内谷胱甘肽含量（含还原和氧化形式）。受精后3 h精子就已穿透，6 h雌性原核形成，12 h发育完全。雄性原核的形成主要发生在授精后9 ~ 12 h。卵母细胞的谷胱甘肽含量随精子穿透时间的延长而下降，并一直维持在较低的水平，直至12 h。电刺激后，卵母细胞活化发生和女性原核已形成的3和6小时，分别。卵母细胞谷胱甘肽含量没有改变，作为卵母细胞激活的结果。当卵母细胞暴露于电脉冲，然后精子，雌性原核形成观察刺激/授精后3小时。在3 - 9 h之间观察到精子穿透。然而，在12小时观察到的雄性原核形成的发生率极低，虽然精子去凝聚发生在一些卵母细胞。电激活后6 h，卵母细胞谷胱甘肽含量没有降低。这些结果表明，猪卵母细胞体外受精后谷胱甘肽含量的变化与电激活引起的变化不同。此外，精子穿透激活的卵母细胞中细胞内谷胱甘肽含量降低似乎是由于精子因子的存在。
Summary Pronuclear formation and intracellular content of glutathione, containing reduced and oxidised forms, in porcine oocytes matured in vitro were determined following insemination and/or electrical stimulation. After insemination, sperm penetration had occurred as early as 3 h and female pronuclei had formed by 6 h with complete development by 12 h. Male pronuclear formation occurred, primarily, between 9 and 12 h after insemination. Glutathione content of the oocytes decreased following sperm penetration and remained at a depressed level until 12 h. After electrical stimulation, oocyte activation had occurred and female pronuclei had formed by 3 and 6 h, respectively. Oocyte glutathione content did not change as a result of oocyte activation. When oocytes were exposed to an electrical pulse and then spermatozoa, female pronuclear formation was observed by 3 h after stimulation/insemination. Sperm penetration was observed between 3 and 9 h. However, the incidence of male pronuclear formation observed at 12 h was extremely low, although sperm decondensation had occurred in some oocytes. Oocyte glutathione content had not decreased by 6 h following electrical activation. These results demonstrate that the changes in glutathione content in porcine oocytes following fertilisation in vitro differ from those due to electrical activation. Further, the decreased intracellular glutathione content in oocytes activated by sperm penetration appears to be due to the presence of a sperm factor.
影响因子：
3.6
作者：
GARDINER, CS;REED, DJ
通讯作者：
REED, DJ