Genome-wide association study identifies QTLs conferring salt tolerance in rice
Genome-wide association study identifies QTLs conferring salt tolerance in rice
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DOI：
10.1111/pbr.12750
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发表时间：
2019-09-30
期刊：
影响因子：
2
通讯作者：
Wan, Jianmin
中科院分区：
文献类型：
--
作者：
An, Hongzhou;Liu, Kai;Wan, Jianmin
水稻是最重要的粮食作物，但对盐胁迫敏感。日益严重的盐碱化正在逐步降低水稻产量，从而促使人们更加呼吁开展研究，以确保可持续的粮食生产。品种的遗传多样性为发现尚未充分利用的耐盐相关基因提供了丰富的资源。本研究以181个小麦品种为核心群体，通过全基因组关联分析（GWAS）检测到54个与耐盐性相关的QTL。检测到17个与干重比（DWR）显著相关的耐盐位点。在7号染色体上的一个新QTL qST-7.19区域，我们鉴定了一个编码多药毒性化合物挤出（MATE）蛋白的候选基因，并利用该候选基因的近等基因系进一步验证了该效应。这些QTL和候选基因为通过分子育种改良耐盐性奠定了基础。
Rice is the most important cereal crop but is sensitive to salinity stress. Increased salinization is progressively reducing rice productivity, escalating calls for research to ensure sustainable food production. The genetic diversity available in cultivars provides a rich resource for discovering genes associated with salt tolerance which has not yet been sufficiently utilized. In this study, 54 QTLs associated with salt tolerance were detected through genome-wide association study (GWAS) using a core population consisting of 181 cultivars. We detected 17 loci significantly associated with dry weight ratio (DWR) for salt tolerance. In the region of a novel QTL qST-7.19 on chromosome 7, we identified a candidate gene encoding a multidrug and toxic compound extrusion (MATE) protein and further validated the effect by using near-isogenic line of the candidate gene. These QTLs and the candidate gene pave the ways for salinity tolerance improvement through molecular breeding.