Chlamydomonas shortens its flagella by activating axonemal disassembly, stimulating IFT particle trafficking, and blocking anterograde cargo loading
Chlamydomonas shortens its flagella by activating axonemal disassembly, stimulating IFT particle trafficking, and blocking anterograde cargo loading
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DOI：
10.1016/j.devcel.2005.07.010
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发表时间：
2005-09-01
影响因子：
11.8
通讯作者：
Snell, WJ
中科院分区：
文献类型：
--
作者：
Pan, JM;Snell, WJ
几乎所有真核细胞都会形成纤毛/鞭毛，将它们维持在遗传指定的长度，并缩短它们。在这里，我们定义了双鞭毛绿藻 Chlarrlydornonas 减数分裂细胞分裂前鞭毛缩短以及响应环境信号的细胞机制。我们表明，鞭毛缩短途径与强制长度控制所必需的短暂缩短途径不同。在鞭毛缩短期间，轴丝的分解受到高于基础速率的刺激，并且鞭毛内运输（IFT）颗粒进入鞭毛的速率增加。此外，进入分解鞭毛的颗粒缺乏货物。因此，鞭毛缩短取决于轴丝动态特性和 IFT 机制之间的相互作用；被触发缩短鞭毛的细胞会激活轴丝的分解，并刺激具有空货物结合位点的 IFT 颗粒进入鞭毛，可用于回收分解的组件。
Almost all eukaryotic cells form cilia/flagella, maintain them at their genetically specified lengths, and shorten them. Here, we define the cellular mechanisms that bring about shortening of flagella prior to meiotic cell division and in response to environmental cues in the biflagellated green alga Chlarrlydornonas. We show that the flagellar shortening pathway is distinct from the one that enforces transient shortening essential for length control. During flagellar shortening, disassembly of the axoneme is stimulated over the basal rate, and the rate of entry into flagella of intraflagellar transport (IFT) particles is increased. Moreover, the particles entering the disassembling flagella lack cargo. Thus, flagellar shortening depends on the interplay between dynamic properties of the axoneme and the IFT machinery; a cell triggered to shorten its flagellum activates disassembly of the axoneme and stimulates entry into the flagellum of IFT particles possessing empty cargo binding sites available to retrieve the disassembled components.