A method for quantitative analysis of spatially variable physiological processes across leaf surfaces
A method for quantitative analysis of spatially variable physiological processes across leaf surfaces
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DOI：
10.1007/s11120-006-9119-z
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发表时间：
2006-11-01
影响因子：
3.7
通讯作者：
DeLucia, Evan H.
中科院分区：
文献类型：
--
作者：
Aldea, Mihai;Frank, Thomas D.;DeLucia, Evan H.
许多生理过程在叶表面是空间可变的。虽然光合作用，气孔导度，基因表达，水运输，和活性氧（ROS）的生产个别叶片的地图很容易获得，量化空间异质性和结合从同一叶，但不同的仪器收集的信息的分析方法没有广泛使用。我们提出了一种新的应用工具，从地理成像领域的多变量分析的生理图像。注册和重新注册，聚类分析和分类的程序提供了一个空间分辨生理数据分析的一般框架。进行了两个实验来说明这种方法的实用性。同时暴露于大气O-3和大豆花叶病毒的大豆叶片的叶绿素荧光和ROS的产生的图像的定量分析显示，PSII的工作量子效率被压抑的叶片的区域也经历了ROS的积累。这种相关性表明了氧化应激和光合作用抑制之间的因果关系。覆盖图的叶表面温度和叶绿素荧光的光抑制处理后表明，PSII的工作量子效率低的地区也经历了气孔导度降低（高温）。虽然这些实验中的每一个都通过叠加用不同仪器收集的独立图像来探索两个过程的协方差，但相同的程序可以用于分析来自多个图像的信息的协方差。从地理图像分析工具的应用，发生在小的空间尺度上的生理过程将有助于揭示机制产生的空间变异叶。
Many physiological processes are spatially variable across leaf surfaces. While maps of photosynthesis, stomatal conductance, gene expression, water transport, and the production of reactive oxygen species (ROS) for individual leaves are readily obtained, analytical methods for quantifying spatial heterogeneity and combining information gathered from the same leaf but with different instruments are not widely used. We present a novel application of tools from the field of geographical imaging to the multivariate analysis of physiological images. Procedures for registration and resampling, cluster analysis, and classification provide a general framework for the analysis of spatially resolved physiological data. Two experiments were conducted to illustrate the utility of this approach. Quantitative analysis of images of chlorophyll fluorescence and the production of ROS following simultaneous exposure of soybean leaves to atmospheric O-3 and soybean mosaic virus revealed that areas of the leaf where the operating quantum efficiency of PSII was depressed also experienced an accumulation of ROS. This correlation suggests a causal relationship between oxidative stress and inhibition of photosynthesis. Overlaying maps of leaf surface temperature and chlorophyll fluorescence following a photoinhibition treatment indicated that areas with low operating quantum efficiency of PSII also experienced reduced stomatal conductance (high temperature). While each of these experiments explored the covariance of two processes by overlaying independent images gathered with different instruments, the same procedures can be used to analyze the covariance of information from multiple images. The application of tools from geographic image analysis to physiological processes occurring over small spatial scales will help reveal the mechanisms generating spatial variation across leaves.