High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host
High-level production of mature active-form Streptomyces mobaraensis transglutaminase via pro-transglutaminase processing using Streptomyces lividans as a host
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DOI：
10.1016/j.bej.2013.02.011
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发表时间：
2013-05-15
影响因子：
3.9
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Noda, Shuhei;Miyazaki, Takaya;Kondo, Akihiko
茂原链霉菌（StreptomycesMobaraensis）转氨酶（MTG）具有广泛的底物特异性和对Ca ~（2+）的不依赖性，是最有用的转氨酶之一。为了实现使用变铅青链霉菌作为宿主的活性形式MTG的有效生产，我们创建了三个载体构建体，所述载体构建体由来源于肉桂链霉菌磷脂酶D基因的信号肽序列（pld信号）、S. cinnamoneus转氨酶和编码成熟MTG的序列，然后获得三个过表达的S. lividans菌株。我们成功地证明了S.变青聚糖可用作有效生产成熟的活性形式MTG的宿主。(C)2013爱思唯尔有限公司版权所有。
Streptomyces mobaraensis transglutaminase (MTG) is one of the most useful transglutaminases due to its rather broad substrate specificity and independence of Ca2+. To achieve efficient production of active-form MTG using Streptomyces lividans as a host, we created three vector constructs consisting of the signal peptide sequence (pld signal) derived from the phospholipase D gene of Streptomyces cinnamoneus, prepro-domain of S. cinnamoneus transglutaminase, and the sequence encoding mature MTG, and then generated three over-expressing S. lividans strains. We successfully demonstrated that S. lividans can be used as a host for the efficient production of mature, active-form MTG. (C) 2013 Elsevier B.V. All rights reserved.