Van Gogh: a new Drosophila tissue polarity gene.
Van Gogh: a new Drosophila tissue polarity gene.
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发表时间：
1998-09
期刊：
影响因子：
3.3
通讯作者：
Job Taylor;N. A. Abramova;J. Charlton;P. Adler
中科院分区：
文献类型：
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作者：
Job Taylor;N. A. Abramova;J. Charlton;P. Adler
梵高基因的突变导致成年果蝇角质层结构的极性改变。在翅膀上，梵高突变导致极性模式改变，这是使卷曲的信号/信号转导途径失活的突变的典型特征。然而，表型与以前看到的不同，因为形成一根以上毛发的翼细胞的数量介于以前看到的典型卷曲状或内翻状突变的数量之间。与梵高参与卷曲的信号/信号转导通路的功能一致，梵高的突变与卷曲和棘突的突变显示出强烈的相互作用。梵高的有丝分裂克隆表现出霸气的细胞非自主性。与卷曲的克隆不同，梵高的克隆改变了克隆近端(以及部分前部和后部)细胞的极性，但不改变远端细胞的极性。与卷曲的克隆人进一步形成对比的是，梵高的克隆人会导致邻近的野生型毛发远离克隆人，而不是指向克隆人。这种反卷曲类型的专横的非自主性，以及在弗莱德和梵高之间看到的强烈的遗传交互作用表明，梵高可能是卷发的非细胞自主功能所必需的。作为对这一可能性的测试，我们在梵高突变背景下诱导了卷曲克隆，并在卷曲突变背景下诱导了梵高克隆。在这两种情况下，霸气的非自主性都被压制了，这与梵高是卷曲的信号所必需的一致。
Mutations in the Van Gogh gene result in the altered polarity of adult Drosophila cuticular structures. On the wing, Van Gogh mutations cause an altered polarity pattern that is typical of mutations that inactivate the frizzled signaling/signal transduction pathway. The phenotype however, differs from those seen previously, as the number of wing cells forming more than one hair is intermediate between that seen previously for typical frizzled-like or inturned-like mutations. Consistent with Van Gogh being involved in the function of the frizzled signaling/signal transduction pathway, Van Gogh mutations show strong interactions with mutations in frizzled and prickle. Mitotic clones of Van Gogh display domineering cell nonautonomy. In contrast to frizzled clones, Van Gogh clones alter the polarity of cells proximal (and in part anterior and posterior) but not distal to the clone. In further contrast to frizzled clones, Van Gogh clones cause neighboring wild-type hairs to point away from rather than toward the clone. This anti-frizzled type of domineering nonautonomy and the strong genetic interactions seen between frizzled and Van Gogh suggested the possibility that Van Gogh was required for the noncell autonomous function of frizzled. As a test of this possibility we induced frizzled clones in a Van Gogh mutant background and Van Gogh clones in a frizzled mutant background. In both cases the domineering nonautonomy was suppressed consistent with Van Gogh being essential for frizzled signaling.