Description of Micrococcus aloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera.
Description of Micrococcus aloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera.
复制标题
芦荟微球菌的描述。
DOI：
10.1099/ijs.0.063339-0
复制
发表时间：
2014
影响因子：
2.8
通讯作者：
Y. Shouche
中科院分区：
文献类型：
--
作者：
O. Prakash;Yogesh S. Nimonkar;H. Munot;Avinash Sharma;V. R. Vemuluri;Mahesh S. Chavadar;Y. Shouche
从采集自印度马哈拉施特拉邦浦那的库拉索芦荟（Aloe vera）的内部肉质叶组织中分离到一种黄色革兰氏染色阳性、不运动、不形成内生孢子、球形内生放线菌，命名为菌株AE-6（T）。菌株AE-6（T）在高盐浓度[10%（w/v）NaCl]、15-41 °C的温度和5-12的pH范围下生长。与云南微球菌YIM 65004（T）的16 S rRNA基因序列相似性最高（99.7%），其次是藤黄微球菌NCTC 2665（T）（99.6%）和内生微球菌YIM 56238（T）（99.0%）。MALDI-TOF/MS核糖体蛋白质谱分析也显示其与M. yunnanensis YIM 65004（T）和M. Luteus NCTC 2665（T）。与微球菌属的其它成员一样，菌株AE-6（T）具有高含量的支链脂肪酸（iso-C15：0和anteiso-C15：0）。MK-8（H2）和MK-8是主要的类异戊二烯醌。细胞壁分析表明，“A2 L-Lys-肽亚基”类型的肽聚糖和核糖是主要的细胞壁糖。DNA G+C含量为70 mol%。AE-6（T）与其来自微球菌属的最近亲缘菌的DNA-DNA杂交结果产生小于70%的值。根据本研究结果，菌株AE-6（T）可以与微球菌属的其他成员明确区分。我们认为它代表微球菌属的一个新种，并建议将其命名为芦荟微球菌，以菌株AE-6（T）（= MCC 2184（T）= DSM 27472（T））作为该种的模式菌株。
A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6(T), was isolated from the inner fleshy leaf tissues of Aloe barbadensis (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6(T) grew at high salt concentrations [10% (w/v) NaCl], temperatures of 15-41 °C and a pH range of 5-12. It showed highest (99.7%) 16S rRNA gene sequence similarity with Micrococcus yunnanensis YIM 65004(T) followed by Micrococcus luteus NCTC 2665(T) (99.6%) and Micrococcus endophyticus YIM 56238(T) (99.0%). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to M. yunnanensis YIM 65004(T) and M. luteus NCTC 2665(T). Like other members of the genus Micrococcus, strain AE-6(T) had a high content of branched chain fatty acids (iso-C15:0 and anteiso-C15:0). MK-8(H2) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an 'A2 L-Lys-peptide subunit' type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA-DNA hybridization of AE-6(T) with its closest relatives from the genus Micrococcus produced a value of less than 70%. Based on the results of this study, strain AE-6(T) could be clearly differentiated from other members of the genus Micrococcus. We propose that it represents a novel species of the genus Micrococcus and suggest the name Micrococcus aloeverae sp. nov., with strain AE-6(T) ( = MCC 2184(T) = DSM 27472(T)) as the type strain of the species.