Assembly of a unique membrane complex in type VI secretion systems of Bacteroidota.
Assembly of a unique membrane complex in type VI secretion systems of Bacteroidota.
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DOI：
10.1038/s41467-023-44426-1
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发表时间：
2024-01-10
影响因子：
16.6
通讯作者：
Durand, Eric
中科院分区：
文献类型：
--
作者：
Bongiovanni, Thibault R.;Latario, Casey J.;Le Cras, Youn;Trus, Evan;Robitaille, Sophie;Swartz, Kerry;Schmidtke, Danica;Vincent, Maxence;Kosta, Artemis;Orth, Jan;Stengel, Florian;Pellarin, Riccardo;Rocha, Eduardo P. C.;Ross, Benjamin D.;Durand, Eric
革兰氏阴性菌的VI型分泌系统（T6 SS）通过毒性效应蛋白的接触依赖性易位来抑制竞争细胞。在变形菌中，T6 SS通过兆道尔顿大小的膜复合物（MC）锚定到细胞被膜。然而，具有T6 SS的拟杆菌门的基因组似乎缺乏编码典型MC组分的同源物的基因。在这里，我们确定了脆弱拟杆菌（tssNQOPR）中的五个基因，这些基因对T6 SS功能至关重要，并编码拟杆菌门特异性MC。我们纯化了这个复合物，使用电子显微镜揭示了它的尺寸，并鉴定了MC组装背后的蛋白质-蛋白质相互作用网络，包括五种TssNQOPR组分的化学计量。TssN蛋白介导拟杆菌MC和保守基板之间的连接。虽然MC基因的内容和组织在拟杆菌门中各不相同，但在T6 SS位点之外没有检测到MC同源物，这表明与假单胞菌门的非同源MC存在古老的互补性和功能趋同性。革兰氏阴性菌的VI型分泌系统（T6 SS）通常通过膜复合物（MC）锚定到细胞被膜，但拟杆菌门似乎缺乏编码典型MC组分的基因。在这里，Bongiovanni等人鉴定了拟杆菌属MC，并研究了其生物起源及其与保守的T6 SS组分的联系。
The type VI secretion system (T6SS) of Gram-negative bacteria inhibits competitor cells through contact-dependent translocation of toxic effector proteins. In Proteobacteria, the T6SS is anchored to the cell envelope through a megadalton-sized membrane complex (MC). However, the genomes of Bacteroidota with T6SSs appear to lack genes encoding homologs of canonical MC components. Here, we identify five genes in Bacteroides fragilis (tssNQOPR) that are essential for T6SS function and encode a Bacteroidota-specific MC. We purify this complex, reveal its dimensions using electron microscopy, and identify a protein-protein interaction network underlying the assembly of the MC including the stoichiometry of the five TssNQOPR components. Protein TssN mediates the connection between the Bacteroidota MC and the conserved baseplate. Although MC gene content and organization varies across the phylum Bacteroidota, no MC homologs are detected outside of T6SS loci, suggesting ancient co-option and functional convergence with the non-homologous MC of Pseudomonadota. The type VI secretion system (T6SS) of Gram-negative bacteria is typically anchored to the cell envelope through a membrane complex (MC), but Bacteroidota appear to lack genes encoding canonical MC components. Here, Bongiovanni et al. identify the Bacteroidota MC and study its biogenesis and connections with the conserved T6SS components.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1073/pnas.2009556117
发表时间：
2020-09-29
影响因子：
11.1
作者：
Garcia-Bayona, Leonor;Coyne, Michael J.;Comstock, Laurie E.
通讯作者：
Comstock, Laurie E.
影响因子：
13.6
作者：
通讯作者：
--
影响因子：
4.5
作者：
Brunet YR;Zoued A;Boyer F;Douzi B;Cascales E
通讯作者：
Cascales E
影响因子：
13.6
作者：
Beckham KSH;Ritter C;Chojnowski G;Ziemianowicz DS;Mullapudi E;Rettel M;Savitski MM;Mortensen SA;Kosinski J;Wilmanns M
通讯作者：
Wilmanns M