Radioimmunoassay for GH-Release Inhibiting Hormone 1
Radioimmunoassay for GH-Release Inhibiting Hormone 1
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GH 释放抑制激素 1 的放射免疫测定
DOI：
10.3181/00379727-148-38631
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发表时间：
1975
影响因子：
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通讯作者：
A. Schally
中科院分区：
文献类型：
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作者：
A. Arimura;Haruko Sato;D. Coy;A. Schally
用戊二醛将合成的生长激素释放抑制激素(GH-RIH)与人血清球蛋白偶联，给兔注射。由此产生的抗血清结合了70%的~(125)I-Tyr1-GH-RIH。未标记的GH-RIH可抑制GH-RIH的结合，且抑制作用呈剂量依赖性，为建立GH-RIH的放射免疫分析方法奠定了基础。最小检测剂量为4pg。大鼠和猪下丘脑提取物的免疫反应性GH-RIH呈线性关系，表明它们含有与GH-RIH难以区分的物质(S)。不同的下丘脑和垂体激素不干扰放射免疫测定，但线性GH-RIH和GH-RIH的环部分有较大的交叉反应，提示抗原决定簇涉及GH-RIH第3-14位或其部分氨基酸序列。血浆蛋白似乎含有免疫上与GH-RIH无法区分的物质(S)，或以非特异性方式干扰GH-RIH的放射免疫分析系统。作者希望对NIAMDD(大鼠垂体激素计划)和Harold Papkoff博士(各种垂体激素)、Merck Sharp和Dohme研究实验室(GH-RH)、雅培实验室(TRH)、Ayerst实验室(GH-RIH)和三共公司(Sankyo，Co.)表示感谢。
Summary The synthetic growth hormone release inhibiting hormone (GH-RIH) was conjugated with human serum globulin using glutaraldehyde and administered to rabbits. An antiserum thus generated bound 70 % of 125I-Tyr1-GH-RIH. The binding was inhibited by unlabelled GH-RIH and the inhibition was dose-related, enabling us to establish a radioimmunoassay method for GH-RIH. The minimum detectable dose was 4 pg. A linearity was demonstrated for im-munoreactive GH-RIH of extracts of rat and pig hypothalami, indicating that they contained substance(s) indistinguishable from GH-RIH. Various hypothalamic and pituitary hormones did not interfere with the radioimmunoassay, but considerable cross-reaction was observed for linear GH-RIH and the ring portion of GH-RIH, suggesting that the antigenic determinant involved the amino acid sequence from position 3-14 of GH-RIH or part of it. Plasma protein appears to contain substance(s) immunologically indistinguishable from GH-RIH or to interfere, in a nonspecific manner, with the radioimmunoassay system for GH-RIH. The authors wish to express our thanks to NIAMDD, Rat Pituitary Hormone Program, and Dr. Harold Papkoff for various pituitary hormones, Merck Sharp and Dohme Research Laboratory for GH-RH, Abbott Laboratory for TRH, Ayerst Laboratory for GH-RIH, and Sankyo, Co., for LH-RH.