Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.
Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.
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DOI：
10.1016/j.bbrc.2016.08.039
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发表时间：
2016-09
影响因子：
3.1
通讯作者：
Jialing Shen;Shinpei Yokota;Hayato Yokoi;Tohru Suzuki
中科院分区：
文献类型：
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作者：
Jialing Shen;Shinpei Yokota;Hayato Yokoi;Tohru Suzuki
已经开发出各种方法将哺乳动物体细胞重编程为多能细胞以及直接将体细胞重编程为其他细胞谱系。我们有兴趣将这些方法应用于鱼类，在这里，我们研究了生殖系特异性基因（vasa，nanos 2，-3）和多能性因子（oct 4，sox 2，c-myc，nanog）的mRNA表达是否在日本青鳉（Oryzias latipes）的体细胞中是可诱导的。我们发现，暴露于致癌物二乙基亚硝胺（DEN）诱导了肠道和再生鳍中vasis的表达。在>50 ppm DEN处理的第5天开始诱导肠道vasain。此外，nanos 2、nanos 3、oct 4、sox 2、klf 4、c-myc和nanog也在一些evasa阳性的肠和再生鳍样品中同时表达。免疫组织化学（IHC）检测到血管阳性细胞在肠道周围的肌肉和伤口表皮，芽基，再生鳍成纤维细胞样细胞。Invasa：GFP转基因青鳉，DEN暴露后，在伤口表皮和再生鳍中的成纤维细胞样细胞中出现绿色荧光蛋白（GFP）荧光，与IHC数据一致。我们的数据表明，生殖细胞的规格和多能性相关基因的mRNA表达可以诱导鱼体细胞暴露于DEN，这表明有效和快速的细胞重编程的鱼体细胞的可能性。
Various methods have been developed to reprogram mammalian somatic cells into pluripotent cells as well as to directly reprogram somatic cells into other cell lineages. We are interested in applying these methods to fish, and here, we examined whether mRNA expression of germline-specific genes (vasa,nanos2,-3) and pluripotency factors (oct4,sox2,c-myc,nanog) is inducible in somatic cells of Japanese medaka (Oryzias latipes). We found that the expression ofvasais induced in the gut and regenerating fin by exposure to a carcinogen, diethylnitrosamine (DEN). Induction ofvasain the gut started on the 5th day of treatment with >50 ppm DEN. In addition,nanos2,-3,oct4,sox2,klf4,c-myc, andnanogwere also expressed simultaneously in somevasa-positive gut and regenerating fin samples. Vasa-positive cells were detected by immunohistochemistry (IHC) in the muscle surrounding the gut and in the wound epidermis, blastema, and fibroblast-like cells in regenerating fin. Invasa:GFPtransgenic medaka, green fluorescent protein (GFP) fluorescence appeared in the wound epidermis and fibroblast-like cells in the regenerating fin following DEN exposure, in agreement with the IHC data. Our data show that mRNA expression of genes relevant to germ cell specification and pluripotency can be induced in fish somatic cells by exposure to DEN, suggesting the possibility of efficient and rapid cell reprogramming of fish somatic cells.