4-Guanidino-n-butyl Syringate (Leonurine, SCM 198) Protects H9c2 Rat Ventricular Cells From Hypoxia-induced Apoptosis
4-Guanidino-n-butyl Syringate (Leonurine, SCM 198) Protects H9c2 Rat Ventricular Cells From Hypoxia-induced Apoptosis
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DOI：
10.1097/fjc.0b013e3181bae160
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发表时间：
2009-11-01
影响因子：
3
通讯作者：
Zhu, Yi-zhun
中科院分区：
文献类型：
--
作者：
Liu, Xin-hua;Chen, Pei-fang;Zhu, Yi-zhun
本研究以益母草中的化学成分益母草碱为基础，以缺氧和血清剥夺诱导的H9 c2大鼠心室肌细胞凋亡为模型，观察化学合成的复方益母草碱对H9 c2大鼠心室肌细胞的保护作用。结果显示，与益母草碱处理相关的细胞活力呈浓度依赖性增加，同时乳酸脱氢酶渗漏到培养基中的能力持续下降。膜联蛋白V-异硫氰酸荧光素阳性细胞的分数增加缺氧，但减少益母草碱。这些变化与抗凋亡基因Bcl-2的表达增加和促凋亡基因Bax的表达减少有关。益母草碱还能降低缺氧引起的细胞内Ca ~（2+）超载。提示益母草碱对H9 c2细胞具有明显的心肌保护作用，其机制可能与抑制细胞内Ca ~（2+）超载和凋亡有关。
In the present study, we examined the ability of it chemically synthesized compound based oil the structure of leonurine, a phytochemical component of Herba leonuri, to protect H9c2 rat ventricular cells from apoptosis induced by hypoxia and serum deprivation, as it model of ischemia. The results revealed a concentration-dependent increase in cell viability associated with leonurine treatment, accompanied by a consistent decline ill lactate dehydrogenase leakage into the culture medium. The fraction of annexin V-fluorescein isothiocyanate-positive cells was increased by hypoxia but reduced by leonurine. These changes were associated with increased expression of the antiapoptotic gene, Bcl-2, and reduced expression of the proapoptotic gene, Bax. Leonurine also reduced the cytosolic Ca2+ overload induced by hypoxia. These results suggest that leonurine elicits potent cardioprotective effects in H9c2 cells, and these effects may be mediated by inhibition of intracellular Ca2+ overload and apoptosis during hypoxia.