Localization of cathepsins D, K, and L in degenerated human intervertebral discs
Localization of cathepsins D, K, and L in degenerated human intervertebral discs
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DOI：
10.1097/00007632-200112150-00007
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发表时间：
2001-12-15
期刊：
影响因子：
3
通讯作者：
Yoshikawa, H
中科院分区：
文献类型：
--
作者：
Ariga, K;Yonenobu, K;Yoshikawa, H
研究设计.组织蛋白酶D、K和L在退变椎间盘中的定位通过荧光化学检测。通过监测退变椎间盘组织中组织蛋白酶的免疫定位，确定组织蛋白酶在椎间盘退变病理机制中的作用。组织蛋白酶D、K和L是有助于在受骨关节炎和类风湿性关节炎影响的关节软骨中观察到的基质破坏的酶。然而，关于这些组织蛋白酶对椎间盘退变的贡献知之甚少。手术时收集的退变椎间盘组织的石蜡包埋切片（12例患者的13个椎间盘）用组织蛋白酶D、K和L的抗体进行化学染色。采用免疫组化方法检测CD68的表达，TRAP染色观察细胞的形态学特征。苏木精和伊红染色显示所有切片均存在明显的变性迹象。组织蛋白酶D和L免疫定位在椎间盘纤维软骨细胞在各个网站表现出退化。组织蛋白酶K被发现在抗酒石酸酸性磷酸酶阳性多核细胞，特别是在软骨终板内的裂缝附近。然而，在纤维环中很少有细胞表达这些组织蛋白酶，这些纤维环保持了胶原纤维的层状结构。在变性部位观察到组织蛋白酶D和L的显著表达。组织蛋白酶D和K定位于软骨终板和椎体之间裂隙的抗酒石酸酸性磷酸酶阳性多核细胞中，这些组织蛋白酶的位点特异性定位表明这些蛋白酶与退行性脊柱疾病中终板分离和纤维环解体有关。
Study Design. Localization of cathepsins D, K, and L in degenerated intervertebral discs was examined by immunohistochemistry.Objectives. To determine the involvement of cathepsins in the pathomechanism of intervertebral disc degeneration by monitoring the immunolocalization of cathepsins in degenerated intervertebral disc tissue.Summary of Background Data. Cathepsins D, K, and L are enzymes that contribute to the matrix destruction seen in the articular cartilage affected by osteoarthritis and rheumatoid arthritis. However, little is known about the contribution of these cathepsins to intervertebral disc degeneration,Methods. Paraffin-embedded sections of degenerated intervertebral disc tissue collected at the time of surgery (13 discs from 12 patients) were immunohistochemically stained with antibodies for cathepsins D, K, and L. For further characterization of the stained cells, immunohistochemical detection of CD68 and TRAP staining were performed.Results. Hematoxylin and eosin staining revealed obvious signs of degeneration in all sections. Cathepsins D and L were immunolocalized in disc fibrochondrocytes at various sites exhibiting degeneration. Cathepsins K were found in tartrate-resistant acid phosphatase-positive multinucleated cells, in particular near the cleft within the cartilaginous endplate. However, few cells were positive for these cathepsins in anulus fibrosus that maintained the lamellar structure of collagen fibers.Conclusions. Marked expression of cathepsins D and L was observed at the site of degeneration. Cathepsins D and K localized in tartrate-resistant acid phosphatase-positive multinucleated cells existed at the cleft between the cartilaginous endplate and vertebral body, The site-specific localization of these cathepsins suggests the association of these proteinases with endplate separation and disorganization of the anulus fibrosus in degenerative spinal disorders.