In silico analyses of maleidride biosynthetic gene clusters.
In silico analyses of maleidride biosynthetic gene clusters.
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DOI：
10.1186/s40694-022-00132-z
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发表时间：
2022-02-17
影响因子：
--
通讯作者：
Bailey AM
中科院分区：
文献类型：
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作者：
Williams K;de Mattos-Shipley KMJ;Willis CL;Bailey AM
马来酸酐是一类结构相似的真菌天然产物，其中许多具有多样的、有效的生物活性。以前的几个maleidride生物合成基因簇的鉴定，以及随后的实验工作，已经确定了“核心”的一套基因，需要构建特征的中型脂环与马来酸酐部分。通过基因组挖掘，这项工作使用这些核心基因发现了10个全新的推定马来酸酐生物合成基因簇，其中包括两个公开的基因组，并编码在以前未测序的epiheveadride生产者Wicklowia aquatica CBS 125634的基因组中。我们对所有已知和推定的马来酸酐生物合成基因簇进行了系统发育分析和比较生物信息学，以进一步了解这些独特的生物合成途径。在线版本包含补充材料，可通过10.1186/s40694-022-00132-z获得。
Maleidrides are a family of structurally related fungal natural products, many of which possess diverse, potent bioactivities. Previous identification of several maleidride biosynthetic gene clusters, and subsequent experimental work, has determined the ‘core’ set of genes required to construct the characteristic medium-sized alicyclic ring with maleic anhydride moieties. Through genome mining, this work has used these core genes to discover ten entirely novel putative maleidride biosynthetic gene clusters, amongst both publicly available genomes, and encoded within the genome of the previously un-sequenced epiheveadride producer Wicklowia aquatica CBS 125634. We have undertaken phylogenetic analyses and comparative bioinformatics on all known and putative maleidride biosynthetic gene clusters to gain further insights regarding these unique biosynthetic pathways. The online version contains supplementary material available at 10.1186/s40694-022-00132-z.