Super-resolution two-photon microscopy via scanning patterned illumination.
Super-resolution two-photon microscopy via scanning patterned illumination.
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DOI：
10.1103/physreve.91.042703
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Zhang HF
中科院分区：
文献类型：
--
作者：
Urban BE;Yi J;Chen S;Dong B;Zhu Y;DeVries SH;Backman V;Zhang HF
We developed two-photon scanning patterned illumination microscopy (2P-SPIM) for super-resolution two-photon imaging. Our approach used a traditional two-photon microscopy setup with temporally modulated excitation to create patterned illumination fields. Combing nine different illuminations and structured illumination reconstruction, super-resolution imaging was achieved in two-photon microscopy. Using 2P-SPIM we achieved a lateral resolution of 141 nm, which represents an improvement by a factor of 1.9 over the corresponding diffraction limit. We further demonstrated super-resolution cellular imaging by 2P-SPIM to image actin cytoskeleton in mammalian cells and three-dimensional imaging in highly scattering retinal tissue.
