Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin
Determination of superoxide dismutase and SOD-mimetic activities by a chemical system: Co2/H2O2/lucigenin
复制标题
DOI：
10.1007/s00216-011-5070-8
复制
发表时间：
2011-05
影响因子：
4.3
通讯作者：
T. V. Zhidkova;E. Proskurnina;É. A. Parfenov;Y. Vladimirov
中科院分区：
文献类型：
--
作者：
T. V. Zhidkova;E. Proskurnina;É. A. Parfenov;Y. Vladimirov
在 Co2+/过氧化氢/光泽精系统中观察到明亮的化学发光。化学发光强度与钴、过氧化氢或光泽精浓度成正比。建议采用化学发光法在 pH 8.5 的钴-过氧化氢-光泽精系统中测定超氧化物歧化酶 (SOD) 活性。在 SOD 浓度范围为 0 至 4.5 nM 之间，相对化学发光强度与 SOD 浓度之间建立了线性依赖性，c1/2= 0.8 nM。在多种组织样本（大鼠血浆、红细胞溶血液和肝线粒体）中测定 SOD 活性。使用了一种在 60°C 下通过热灭活干扰蛋白来制备组织样品的技术。该方法已成功应用于 SOD 模拟物效率的比较。
The bright chemiluminescence has been observed in the system: Co2+/hydrogen peroxide/lucigenin. The chemiluminescence intensity was directly proportional to either cobalt, hydrogen peroxide, or lucigenin concentrations. A procedure of determination of superoxide dismutase (SOD) activity by the chemiluminescence method in the cobalt–hydrogen peroxide–lucigenin system at pH 8.5 is suggested. A linear dependence was established between a relative chemiluminescence intensity and SOD concentration in the range of SOD concentrations between 0 and 4.5 nM,c1/2= 0.8 nM. The determination of SOD activity was performed in several tissue samples (rat plasma, erythrocyte hemolysate, and liver mitochondria). A technique of tissue sample preparation with the use of thermal inactivation of interfering proteins at 60 °C was used. The method was successfully applied for comparison of the efficiency of SOD mimetics.